ArticleApplied and environmental microbiology2014
Response of Xylella fastidiosa to zinc: decreased culturability, increased exopolysaccharide production, and formation of resilient biofilms under flow conditions.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed.
- Heavy Metal and Petroleum Hydrocarbon Contaminants Promote Resistance and Biofilm Formation inMicroorganisms · 2025Article
- Microfluidic Array Enables Rapid Testing of Natural Compounds AgainstPlants (Basel, Switzerland) · 2025Article
- Influence of Copper onMicroorganisms · 2024Article
- Calcium modulation of bacterial wilt disease on potato.Applied and environmental microbiology · 2024Article
- Article
- Transcriptional profiling of Xanthomonas campestris pv. campestris in viable but nonculturable state.BMC genomics · 2023Article
- The Role of RelA and SpoT on ppGpp Production, Stress Response, Growth Regulation, and Pathogenicity in Xanthomonas campestris pv.Microbiology spectrum · 2021Article
- Progress towards Sustainable Control ofPathogens (Basel, Switzerland) · 2021Review
- Assessment of the Hyperspectral Data Analysis as a Tool to DiagnosePlants (Basel, Switzerland) · 2021Article
- Further In Vitro Assessment and Mid-Term Evaluation of Control Strategy ofPathogens (Basel, Switzerland) · 2021Article
- HPLC-HRMS Global Metabolomics Approach for the Diagnosis of "Olive Quick Decline Syndrome" Markers in Olive Trees Leaves.Metabolites · 2021Article
- Antimicrobial Peptides With Antibiofilm Activity AgainstFrontiers in microbiology · 2021Article
- Zinkicide Is a ZnO-Based Nanoformulation with Bactericidal Activity against Liberibacter crescens in Batch Cultures and in Microfluidic Chambers Simulating Plant Vascular Systems.Applied and environmental microbiology · 2020Article
- Screening and identification of BP100 peptide conjugates active against Xylella fastidiosa using a viability-qPCR method.BMC microbiology · 2020Article
- Genome-Scale Investigation of the Metabolic Determinants Generating Bacterial Fastidious Growth.mSystems · 2020Article
- Calcium transcriptionally regulates movement, recombination and other functions of Xylella fastidiosa under constant flow inside microfluidic chambers.Microbial biotechnology · 2020Article
- Non-Lethal Effects ofMicroorganisms · 2019Article
- Article
- Induction and Resuscitation of the Viable but Non-culturable (VBNC) State inFrontiers in microbiology · 2019Article
- Selection and dissemination of antimicrobial resistance in Agri-food production.Antimicrobial resistance and infection control · 2019Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacterial plant pathogen Xylella fastidiosa produces biofilm that accumulates in the host xylem vessels, affecting disease development in various crops and bacterial acquisition by insect vectors. Biofilms are sensitive to the chemical composition of the environment, and mineral elements being transported in the xylem are of special interest for this pathosystem. Here, X. fastidiosa liquid cultures were supplemented with zinc and compared with nonamended cultures to determine the effects of Zn on growth, biofilm, and exopolysaccharide (EPS) production under batch and flow culture conditions. The results show that Zn reduces growth and biofilm production under both conditions. However, in microfluidic chambers under liquid flow and with constant bacterial supplementation (closer to conditions inside the host), a dramatic increase in biofilm aggregates was seen in the Zn-amended medium. Biofilms formed under these conditions were strongly attached to surfaces and were not removed by medium flow. This phenomenon was correlated with increased EPS production in stationary-phase cells grown under high Zn concentrations. Zn did not cause greater adhesion to surfaces by individual cells. Additionally, viability analyses suggest that X. fastidiosa may be able to enter the viable but nonculturable state in vitro, and Zn can hasten the onset of this state. Together, these findings suggest that Zn can act as a stress factor with pleiotropic effects on X. fastidiosa and indicate that, although Zn could be used as a bactericide treatment, it could trigger the undesired effect of stronger biofilm formation upon reinoculation events.
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