ArticleApplied and environmental microbiology2014
Lactobacillus buchneri genotyping on the basis of clustered regularly interspaced short palindromic repeat (CRISPR) locus diversity.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 52 citations in OpenAlex.
- Application and Research Prospects of CRISPR/Cas Gene Editing Technology in Lactic Acid Bacteria.Microorganisms · 2026Review
- Drug-food Interactions in the Era of Molecular Big Data, Machine Intelligence, and Personalized Health.Recent advances in food, nutrition & agriculture · 2022Review
- Article
- Theragnostic application of nanoparticle and CRISPR against food-borne multi-drug resistant pathogens.Materials today. Bio · 2022Review
- Characterization of CRISPR-Cas systems inMicrobial genomics · 2022Article
- Genome Features andBioMed research international · 2021Article
- Characterization of a Type II-A CRISPR-Cas System inmSphere · 2020Article
- The Diversity of the CRISPR-Cas System and Prophages Present in the Genome Reveals the Co-evolution ofFrontiers in microbiology · 2020Article
- Comparative genomics of eight Lactobacillus buchneri strains isolated from food spoilage.BMC genomics · 2019Article
- CRISPR Visualizer: rapid identification and visualization of CRISPR loci via an automated high-throughput processing pipeline.RNA biology · 2019Article
- Investigation of direct repeats, spacers and proteins associated with clustered regularly interspaced short palindromic repeat (CRISPR) system of Vibrio parahaemolyticus.Molecular genetics and genomics : MGG · 2019Article
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- Article
- Bacterial CRISPR Regions: General Features and their Potential for Epidemiological Molecular Typing Studies.The open microbiology journal · 2018Review
- Incomplete Selective Sweeps ofFrontiers in microbiology · 2018Article
- Characterization and Exploitation of CRISPR Loci inFrontiers in microbiology · 2017Article
- The yajC gene from Lactobacillus buchneri and Escherichia coli and its role in ethanol tolerance.Journal of industrial microbiology & biotechnology · 2016Article
- Diversity of CRISPR-Cas immune systems and molecular machines.Genome biology · 2015Review
- Differential Distribution of Type II CRISPR-Cas Systems in Agricultural and Nonagricultural Campylobacter coli and Campylobacter jejuni Isolates Correlates with Lack of Shared Environments.Genome biology and evolution · 2015Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clustered regularly interspaced short palindromic repeats (CRISPR) in combination with associated sequences (cas) constitute the CRISPR-Cas immune system, which uptakes DNA from invasive genetic elements as novel "spacers" that provide a genetic record of immunization events. We investigated the potential of CRISPR-based genotyping of Lactobacillus buchneri, a species relevant for commercial silage, bioethanol, and vegetable fermentations. Upon investigating the occurrence and diversity of CRISPR-Cas systems in Lactobacillus buchneri genomes, we observed a ubiquitous occurrence of CRISPR arrays containing a 36-nucleotide (nt) type II-A CRISPR locus adjacent to four cas genes, including the universal cas1 and cas2 genes and the type II signature gene cas9. Comparative analysis of CRISPR spacer content in 26 L. buchneri pickle fermentation isolates associated with spoilage revealed 10 unique locus genotypes that contained between 9 and 29 variable spacers. We observed a set of conserved spacers at the ancestral end, reflecting a common origin, as well as leader-end polymorphisms, reflecting recent divergence. Some of these spacers showed perfect identity with phage sequences, and many spacers showed homology to Lactobacillus plasmid sequences. Following a comparative analysis of sequences immediately flanking protospacers that matched CRISPR spacers, we identified a novel putative protospacer-adjacent motif (PAM), 5'-AAAA-3'. Overall, these findings suggest that type II-A CRISPR-Cas systems are valuable for genotyping of L. buchneri.
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