ArticleApplied and environmental microbiology2014
Evaluation of phytate-degrading Lactobacillus culture administration to broiler chickens.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.
- Probiotics and Their Functional Role in Mitigating Antinutrient Effects In Vivo-A Systematic Review and Meta-Analysis.Comprehensive reviews in food science and food safety · 2026Pooled it
- The Potential Value of Probiotics after Dental Implant Placement.Microorganisms · 2023Review
- Wide-genome selection of lactic acid bacteria harboring genes that promote the elimination of antinutritional factors.Frontiers in plant science · 2023Article
- Efficacy of Various Feed Additives on Performance, Nutrient Digestibility, Bone Quality, Blood Constituents, and Phosphorus Absorption and Utilization of Broiler Chickens Fed Low Phosphorus Diet.Animals : an open access journal from MDPI · 2022Article
- Impact of Ag Nanoparticles (AgNPs) and Multimicrobial Preparation (EM) on the Carcass, Mineral, and Fatty Acid Composition ofAnimals : an open access journal from MDPI · 2021Article
- Effect of almond hulls as an alternative ingredient on broiler performance, nutrient digestibility, and cecal microbiota diversity.Poultry science · 2021Article
- Inclusion of Lactobacillus salivarius strain revealed a positive effect on improving growth performance, fecal microbiota and immunological responses in chicken.Archives of microbiology · 2021Article
- Transcriptome Analysis Reveals the Genes Involved in Growth and Metabolism in Muscovy Ducks.BioMed research international · 2021Article
- Probiotic Validation of a Non-native, Thermostable, Phytase-Producing Bacterium: Streptococcus thermophilus.Current microbiology · 2020Article
- Genetic and Phenotypic Characteristics of a Multi-strain Probiotic for Broilers.Current microbiology · 2020Article
- Assembly of hundreds of novel bacterial genomes from the chicken caecum.Genome biology · 2020Article
- New crosstalk between probiotics Lactobacillus plantarum and Bacillus subtilis.Scientific reports · 2019Article
- Dephytinizing and Probiotic Potentials of Saccharomyces cerevisiae (NCIM 3662) Strain for Amelioration of Nutritional Quality of Functional Foods.Probiotics and antimicrobial proteins · 2019Article
- Biological Activities of Lactose-Based Prebiotics and Symbiosis with Probiotics on Controlling Osteoporosis, Blood-Lipid and Glucose Levels.Medicina (Kaunas, Lithuania) · 2018Review
- Use of Lactobacilli in Cereal-Legume Fermentation and as Potential Probiotics towards Phytate Hydrolysis.Probiotics and antimicrobial proteins · 2018Article
- Microbial degradation of3 Biotech · 2018Article
- Probiotics or pro-healers: the role of beneficial bacteria in tissue repair.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society · 2017Review
- Article
- Phytase-Producing Potential and Other Functional Attributes of Lactic Acid Bacteria Isolates for Prospective Probiotic Applications.Probiotics and antimicrobial proteins · 2016Article
- Coexpression and secretion of endoglucanase and phytase genes in Lactobacillus reuteri.International journal of molecular sciences · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Probiotics have been demonstrated to promote growth, stimulate immune responses, and improve food safety of poultry. While widely used, their effectiveness is mixed, and the mechanisms through which they contribute to poultry production are not well understood. Microbial phytases are increasingly supplemented in feed to improve digestibility and reduce antinutritive effects of phytate. The microbial origin of these exogenous enzymes suggests a potentially important mechanism of probiotic functionality. We investigated phytate degradation as a novel probiotic mechanism using recombinant Lactobacillus cultures expressing Bacillus subtilis phytase. B. subtilis phyA was codon optimized for expression in Lactobacillus and cloned into the expression vector pTRK882. The resulting plasmid, pTD003, was transformed into Lactobacillus acidophilus, Lactobacillus gallinarum, and Lactobacillus gasseri. SDS-PAGE revealed a protein in the culture supernatants of Lactobacillus pTD003 transformants with a molecular weight similar to that of the B. subtilis phytase. Expression of B. subtilis phytase increased phytate degradation of L. acidophilus, L. gasseri, and L. gallinarum approximately 4-, 10-, and 18-fold over the background activity of empty-vector transformants, respectively. Phytase-expressing L. gallinarum and L. gasseri were administered to broiler chicks fed a phosphorus-deficient diet. Phytase-expressing L. gasseri improved weight gain of broiler chickens to a level comparable to that for chickens fed a control diet adequate in phosphorus, demonstrating proof of principle that administration of phytate-degrading probiotic cultures can improve performance of livestock animals. This will inform future studies investigating whether probiotic cultures are able to provide both the performance benefits of feed enzymes and the animal health and food safety benefits traditionally associated with probiotics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.