ArticleMemorias do Instituto Oswaldo Cruz2013
Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp.
Article in Memorias do Instituto Oswaldo Cruz, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.
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Who cites it
8 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review.Memorias do Instituto Oswaldo Cruz · 2022Pooled it
- Signal peptide variation in cyst lectins as a potential marker for pathogenic Acanthamoeba spp.Parasites & vectors · 2026Article
- Experimental Granulomatous Amebic Encephalitis Caused byTropical medicine and infectious disease · 2024Article
- Plants of the family Lamiaceae as a source of therapeutic agents against Acanthamoeba infections.Memorias do Instituto Oswaldo Cruz · 2024Review
- Experimental keratitis induced in rat by Acanthamoeba from distinct morphological groups/genotypes: a histological and immunohistochemical evaluation.Parasitology research · 2023Article
- Comparative proteomic profiling of newly acquired, virulent and attenuated Neoparamoeba perurans proteins associated with amoebic gill disease.Scientific reports · 2021Article
- Long-TermJournal of immunology research · 2021Article
- Extracellular protease profile of Acanthamoeba after prolonged axenic culture and after interaction with MDCK cells.Parasitology research · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prolonged culturing of many microorganisms leads to the loss of virulence and a reduction of their infective capacity. However, little is known about the changes in the pathogenic strains of Acanthamoeba after long culture periods. Our study evaluated the effect of prolonged culturing on the invasiveness of different isolates of Acanthamoeba in an in vivo rat model. ATCC strains of Acanthamoeba, isolates from the environment and clinical cases were evaluated. The in vivo model was effective in establishing the infection and differentiating the pathogenicity of the isolates and re-isolates. The amoebae cultured in the laboratory for long periods were less virulent than those that were recently isolated, confirming the importance of passing Acanthamoeba strains in animal models.
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