ArticleThe Journal of clinical investigation2013
Mice generated by in vitro fertilization exhibit vascular dysfunction and shortened life span.
Article in The Journal of clinical investigation, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
82 citing papers in PubMed, 2 syntheses or guidelines pooled it, 178 citations in OpenAlex.
- Infertility treatment and offspring blood pressure-a systematic review and meta-analysis.Human reproduction update · 2025Pooled it
- Offspring physiology following the use of IVM, IVF and ICSI: a systematic review and meta-analysis of animal studies.Human reproduction update · 2023Pooled it
- Trial
- Comparison of Histone H3K4me3 between IVF and ICSI Technologies and between Boy and Girl Offspring.International journal of molecular sciences · 2021Trial
- The effects of vitamin D supplementation on metabolic profiles and gene expression of insulin and lipid metabolism in infertile polycystic ovary syndrome candidates for in vitro fertilization.Reproductive biology and endocrinology : RB&E · 2018Trial
- Effect of Perinatal and Maternal Factors on Blood Pressure and Adiposity at Age 3.Journal of clinical hypertension (Greenwich, Conn.) · 2026Observational
- Mouse placentae generated by in vitro fertilization exhibit altered gene expression, activated hypoxia responses, and reduced fitness†.Biology of reproduction · 2026Article
- Transgenerational Impact of Environmental Change.Advances in experimental medicine and biology · 2026Review
- Impact of embryo culture and day of embryo transfer on the cardiometabolic health of adult mice†.Biology of reproduction · 2025Article
- The effect of in vitro fertilization on the cardiac health of mice.BMC cardiovascular disorders · 2025Article
- Review
- Allergic Disposition of IVF-Conceived Mice.International journal of molecular sciences · 2024Article
- Sendai virus-mediated RNA delivery restores fertility to congenital and chemotherapy-induced infertile female mice.PNAS nexus · 2024Article
- Microbiome in Female Reproductive Health: Implications for Fertility and Assisted Reproductive Technologies.Genomics, proteomics & bioinformatics · 2024Review
- Ultrasound evaluation of the cardiovascular system in offspring conceived through assisted reproductive technology.Journal of assisted reproduction and genetics · 2024Review
- Blood pressure and lipid profiles in children born after ART with frozen embryo transfer.Human reproduction open · 2024Article
- Cardiovascular health of offspring conceived by assisted reproduction technology: a comprehensive review.Frontiers in cardiovascular medicine · 2024Review
- Eclampsia,Translational pediatrics · 2023Article
- Left ventricular systolic function in subjects conceived through assisted reproductive technologies.Frontiers in cardiovascular medicine · 2023Article
- Study protocol for a Developmental Epidemiological Study of Children born through Reproductive Technologies (DESCRT).Human reproduction open · 2023Review
22 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Children conceived by assisted reproductive technologies (ART) display a level of vascular dysfunction similar to that seen in children of mothers with preeclamspia. The long-term consequences of ART-associated vascular disorders are unknown and difficult to investigate in healthy children. Here, we found that vasculature from mice generated by ART display endothelial dysfunction and increased stiffness, which translated into arterial hypertension in vivo. Progeny of male ART mice also exhibited vascular dysfunction, suggesting underlying epigenetic modifications. ART mice had altered methylation at the promoter of the gene encoding eNOS in the aorta, which correlated with decreased vascular eNOS expression and NO synthesis. Administration of a deacetylase inhibitor to ART mice normalized vascular gene methylation and function and resulted in progeny without vascular dysfunction. The induction of ART-associated vascular and epigenetic alterations appeared to be related to the embryo environment; these alterations were possibly facilitated by the hormonally stimulated ovulation accompanying ART. Finally, ART mice challenged with a high-fat diet had roughly a 25% shorter life span compared with control animals. This study highlights the potential of ART to induce vascular dysfunction and shorten life span and suggests that epigenetic alterations contribute to these problems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.