ArticleCryobiology2014
Membrane permeability of the human granulocyte to water, dimethyl sulfoxide, glycerol, propylene glycol and ethylene glycol.
Article in Cryobiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 62 citations in OpenAlex.
- Water and cryoprotectant permeability of mature equine oocytes: experimental measurements and in silico predictions.Scientific reports · 2026Article
- Cryopreservation of biological materials: applications and economic perspectives.In vitro cellular & developmental biology. Animal · 2026Review
- FlowCLOc, a New Tool for Selecting the Most Appropriate Antibodies in Flow Cytometry.International journal of molecular sciences · 2026Article
- High-throughput evaluation of cryoprotective agents for mixture effects that reduce toxicity.Cryobiology · 2025Article
- Structural and Viability Assessment of Bovine Corneas Preserved at an Accessible Low-Temperature (-20 °C) for Eye Irritation Models.ACS omega · 2025Article
- High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents.Scientific reports · 2025Article
- High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents.bioRxiv : the preprint server for biology · 2024Article
- Temperature Dependence of Membrane Permeability Parameters for Five Cell Types Using Nonideal Thermodynamic Assumptions to Mathematically Model Cryopreservation Protocols.The journal of physical chemistry. B · 2024Article
- A high-field cellular DNP-supported solid-state NMR approach to study proteins with sub-cellular specificity.Chemical science · 2023Article
- Article
- The Role of Aquaporin 7 in the Movement of Water and Cryoprotectants in Bovine In Vitro Matured Oocytes.Animals : an open access journal from MDPI · 2022Article
- Effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches.Scientific reports · 2021Article
- Influence of Sucrose on the Efficiency of Cryopreservation of Human Umbilical Cord-Derived Multipotent Stromal Cells with the Use of Various Penetrating Cryoprotectants.Bulletin of experimental biology and medicine · 2021Article
- Rapid quantification of multi-cryoprotectant toxicity using an automated liquid handling method.Cryobiology · 2021Article
- Principles of Ice-Free Cryopreservation by Vitrification.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A single-cell identification and capture chip for automatically and rapidly determining hydraulic permeability of cells.Analytical and bioanalytical chemistry · 2020Article
- Implications of variability in cell membrane permeability for design of methods to remove glycerol from frozen-thawed erythrocytes.Cryobiology · 2020Article
- Determination of the Membrane Transport Properties of Jurkat Cells with a Microfluidic Device.Micromachines · 2019Article
- New Approaches to Cryopreservation of Cells, Tissues, and Organs.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2019Review
- Cell Swelling Induced by the Antimalarial KAE609 (Cipargamin) and Other PfATP4-Associated Antimalarials.Antimicrobial agents and chemotherapy · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Granulocytes are currently transfused as soon as possible after collection because they rapidly deteriorate after being removed from the body. This short shelf life complicates the logistics of granulocyte collection, banking, and safety testing. Cryopreservation has the potential to significantly increase shelf life; however, cryopreservation of granulocytes has proven to be difficult. In this study, we investigate the membrane permeability properties of human granulocytes, with the ultimate goal of using membrane transport modeling to facilitate development of improved cryopreservation methods. We first measured the equilibrium volume of human granulocytes in a range of hypo- and hypertonic solutions and fit the resulting data using a Boyle-van't Hoff model. This yielded an isotonic cell volume of 378 μm(3) and an osmotically inactive volume of 165 μm(3). To determine the permeability of the granulocyte membrane to water and cryoprotectant (CPA), cells were injected into well-mixed CPA solution while collecting volume measurements using a Coulter Counter. These experiments were performed at temperatures ranging from 4 to 37°C for exposure to dimethyl sulfoxide, glycerol, ethylene glycol, and propylene glycol. The best-fit water permeability was similar in the presence of all of the CPAs, with an average value at 21°C of 0.18 μmatm(-1)min(-1). The activation energy for water transport ranged from 41 to 61 kJ/mol. The CPA permeability at 21°C was 6.4, 1.0, 8.4, and 4.0 μm/min for dimethyl sulfoxide, glycerol, ethylene glycol, and propylene glycol, respectively, and the activation energy for CPA transport ranged between 59 and 68 kJ/mol.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.