Evidence map›Paper›PMID 24269519›Full record

ArticleBone2014

Nanoscale changes in collagen are reflected in physical and mechanical properties of bone at the microscale in diabetic rats.

Max A Hammond, Maxime A Gallant, David B Burr, Joseph M Wallace

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bone, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.

0numbers the graph read from it
0cells of the map it votes in
53citing papers in PubMed
8.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

53 citing papers in PubMed, 90 citations in OpenAlex.

  1. Review
  2. The Impact of Ageing on Fibrillar Collagens.Sub-cellular biochemistry · 2026
    Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Role of the Mitochondrial Permeability Transition in Bone Metabolism and Aging.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023
    Article
  10. Article
  11. Article
  12. Article
  13. The relative contribution of bone microarchitecture and matrix composition to implant fixation strength in rats.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022
    Article
  14. Review
  15. Review
  16. Article
  17. Review
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  19. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Max A HammondWeldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.
Maxime A GallantDepartment of Anatomy and Cell Biology, Indiana University School of Medicine, IN, USA.
David B BurrDepartment of Anatomy and Cell Biology, Indiana University School of Medicine, IN, USA; Department of Biomedical Engineering, Indiana University-Purdue University at Indianapolis, IN, USA.
Joseph M WallaceWeldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA; Department of Orthopaedic Surgery, Indiana University School of Medicine, IN, USA; Department of Biomedical Engineering, Indiana University-Purdue University at Indianapolis, IN, USA. Electronic address: jmwalla@iupui.edu.
Purdue University West Lafayette · USIndiana University · USIndiana University School of Medicine

Funding

Remodeling Suppression--Damage and bone toughnessR01AR047838 · NIAMS · INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS · PI BURR, DAVID B · 2002 to 2011
$3.6M
NIAMS NIH HHS AR 047838NIAMS NIH HHS R01 AR047838
6 · The paper itself

Abstract

Diabetes detrimentally affects the musculoskeletal system by stiffening the collagen matrix due to increased advanced glycation end products (AGEs). In this study, tibiae and tendon from Zucker diabetic Sprague-Dawley (ZDSD) rats were compared to Sprague-Dawley derived controls (CD) using Atomic Force Microscopy. ZDSD and CD tibiae were compared using Raman Spectroscopy and Reference Point Indentation (RPI). ZDSD bone had a significantly different distribution of collagen D-spacing than CD (p=0.015; ZDSD n=294 fibrils; CD n=274 fibrils) which was more variable and shifted to higher values. This shift between ZDSD and CD D-spacing distribution was more pronounced in tendon (p<0.001; ZDSD n=350; CD n=371). Raman revealed significant increases in measures of bone matrix mineralization in ZDSD (PO4(3-) ν1/Amide I p=0.008; PO4(3-) ν1/CH2 wag p=0.047; n=5 per group) despite lower bone mineral density (aBMD) and ash fraction indicating diabetes may preferentially reduce the Raman signature of collagen. Decreased indentation distance increase (p=0.010) and creep indentation distance (p=0.040) measured by RPI (n=9 per group) in ZDSD rats suggest a matrix more resistant to indentation under the high stresses associated with RPI at this length scale. There were significant correlations between Raman and RPI measurements in the ZDSD population (n=18 locations) but not the CD population (n=16 locations) indicating that while RPI is relatively unaffected by biological noise, it is sensitive to disease-induced compositional changes. In conclusion, diabetes in the ZDSD rat causes changes to the nanoscale morphology of collagen that result in compositional and mechanical effects in bone at the microscale.

Indexed as

AnimalsBiomechanical PhenomenaBone and BonesCollagenDiabetes Mellitus, ExperimentalMaleMicroscopy, Atomic ForceNanostructuresRatsRats, Sprague-DawleyRats, ZuckerSpectrum Analysis, RamanTibiaCollagenAdvanced glycation end productsAtomic Force MicroscopyD-spacingRaman SpectroscopyReference Point IndentationZucker diabetic Sprague–Dawley

Identifiers

PMID24269519
PMCPMC3944921
OpenAlexW2024585892

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.