ArticleInsect biochemistry and molecular biology2014
Changes in transcript abundance for cuticular proteins and other genes three hours after a blood meal in Anopheles gambiae.
Article in Insect biochemistry and molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
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- Molecular drivers of insecticide resistance in the Sahelo-Sudanian populations of a major malaria vector Anopheles coluzzii.BMC biology · 2023Article
- A globalBMC bioinformatics · 2022Article
- Ornithine decarboxylase deficiency critically impairs nitrogen metabolism and survival in Aedes aegypti mosquitoes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Aedes aegypti dyspepsia encodes a novel member of the SLC16 family of transporters and is critical for reproductive fitness.PLoS neglected tropical diseases · 2021Article
- bsAS, an antisense long non-coding RNA, essential for correct wing development through regulation of blistered/DSRF isoform usage.PLoS genetics · 2020Article
- Proteomics reveals localization of cuticular proteins in Anopheles gambiae.Insect biochemistry and molecular biology · 2019Article
- A Complex Lens for a Complex Eye.Integrative and comparative biology · 2017Article
- Serine protease-related proteins in the malaria mosquito, Anopheles gambiae.Insect biochemistry and molecular biology · 2017Article
- The cuticular nature of corneal lenses in Drosophila melanogaster.Development genes and evolution · 2017Article
- Properties of the cuticular proteins of Anopheles gambiae as revealed by serial extraction of adults.PloS one · 2017Article
- Immunolocalization of cuticular proteins in Johnston's organ and the corneal lens of Anopheles gambiae.Arthropod structure & development · 2016Article
- Characterization of the transcriptomes and cuticular protein gene expression of alate adult, brachypterous neotenic and adultoid reproductives of Reticulitermes labralis.Scientific reports · 2016Article
- Ascarosides coordinate the dispersal of a plant-parasitic nematode with the metamorphosis of its vector beetle.Nature communications · 2016Article
- Distribution of cuticular proteins in different structures of adult Anopheles gambiae.Insect biochemistry and molecular biology · 2016Article
- Age and prior blood feeding of Anopheles gambiae influences their susceptibility and gene expression patterns to ivermectin-containing blood meals.BMC genomics · 2015Article
- The CPCFC cuticular protein family: Anatomical and cuticular locations in Anopheles gambiae and distribution throughout Pancrustacea.Insect biochemistry and molecular biology · 2015Article
- Temporal and spatial expression of cuticular proteins of Anopheles gambiae implicated in insecticide resistance or differentiation of M/S incipient species.Parasites & vectors · 2014Article
- Insights into the epigenomic landscape of the human malaria vector Anopheles gambiae.Frontiers in genetics · 2014Article
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Authors and funding
4 authors at 2 institutions in 1 country.
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Abstract
Numerous studies have examined changes in transcript levels after Anopheles gambiae takes a blood meal. Marinotti et al. (2006) used microarrays and reported massive changes in transcript levels 3 h after feeding (BF3h) compared to non-blood fed (NBF). We were intrigued by the number of transcripts for structural cuticular proteins (CPs) that showed such major differences in levels and employed paired-end (50 bp) RNA-seq technology to compare whole body transcriptomes from 5-day-old females NBF and BF3h. We detected transcripts for the majority of CPs (164/243) but levels of only 12 were significantly altered by the blood meal. While relative transcript levels of NBF females were somewhat similar to the microarray data, there were major differences in BF3h animals, resulting in levels of many transcripts, both for CPs and other genes changing in the opposite direction. We compared our data also to other studies done with both microarrays and RNA-seq. Findings were consistent that a small number of CP genes have transcripts that persist even in 5-day-old adults. Some of these transcripts showed diurnal rhythms (Rund et al., 2013; Rinker et al., 2013). In situ hybridization revealed that transcripts for several of these CP genes were found exclusively or predominantly in the eye. Transcripts other than for CPs that changed in response to blood-feeding were predominantly expressed in midgut and Malpighian tubules. Even in these tissues, genes responsible for proteins with similar functions, such as immunity or digestion, responded differently, with transcript levels for some rising and others falling. These data demonstrate that genes coding for some CPs are dynamic in expression even in adults and that the response to a blood meal is rapid and precisely orchestrated.
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