ArticleMutation research2014
Effect of dNTP pool alterations on fidelity of leading and lagging strand DNA replication in E. coli.
Article in Mutation research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Escherichia coli DNA replication: the old model organism still holds many surprises.FEMS microbiology reviews · 2024Review
- Role of RNase H enzymes in maintaining genome stability in Escherichia coli expressing a steric-gate mutant of pol VDNA repair · 2019Article
- Replication fidelity in E. coli: Differential leading and lagging strand effects for dnaE antimutator alleles.DNA repair · 2019Article
- Determinants of Base-Pair Substitution Patterns Revealed by Whole-Genome Sequencing of DNA Mismatch Repair DefectiveGenetics · 2018Article
- Bacterial EndoMS/NucS acts as a clamp-mediated mismatch endonuclease to prevent asymmetric accumulation of replication errors.Nucleic acids research · 2018Article
- SiSTL2 Is Required for Cell Cycle, Leaf Organ Development, Chloroplast Biogenesis, and Has Effects on CFrontiers in plant science · 2018Article
- Optimization of amino acid replacement costs by mutational pressure in bacterial genomes.Scientific reports · 2017Article
- Role of Ribonucleotide Reductase in Bacillus subtilis Stress-Associated Mutagenesis.Journal of bacteriology · 2017Article
- Colon cancer-associated mutator DNA polymerase δ variant causes expansion of dNTP pools increasing its own infidelity.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Suppression of the E. coli SOS response by dNTP pool changes.Nucleic acids research · 2015Article
- Deoxyribonucleotides as genetic and metabolic regulators.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The fidelity with which organisms replicate their chromosomal DNA is of considerable interest. Detailed studies in the bacterium Escherichia coli have indicated that the fidelity of leading- and lagging-strand DNA replication is not the same, based on experiments in which the orientation of certain mutational targets on the chromosome was inverted relative to the movement of the replication fork: different mutation rates for several base-pair substitutions were observed depending on this orientation. While these experiments are indicative of differential replication fidelity in the two strands, a conclusion whether leading or lagging strand is the more accurate depends on knowledge of the primary mispairing error responsible for the base substitutions in question. A broad analysis of in vitro base-pairing preferences of DNA polymerases led us to propose that lagging-strand is the more accurate strand. In the present work, we present more direct in vivo evidence in support of this proposal. We determine the orientation dependence of mutant frequencies in ndk and dcd strains, which carry defined dNTP pool alterations. As these pool alterations lead to predictable effects on the array of possible mispairing errors, they mark the strands in which the observed errors occur. The combined results support the proposed higher accuracy of lagging-strand replication in E. coli.
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Registered trials
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