Evidence map›Paper›PMID 24268700›Full record

ArticleDevelopmental cell2013

Spindle formation in the mouse embryo requires Plk4 in the absence of centrioles.

Paula A Coelho, Leah Bury, Bedra Sharif, Maria G Riparbelli, Jingyan Fu, Giuliano Callaini, David M Glover, Magdalena Zernicka-Goetz

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
3.6field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 71 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. The Centriole's Role in Miscarriages.Frontiers in cell and developmental biology · 2022
    Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Frontiers in cell and developmental biology · 2020
    Article
  13. Article
  14. Review
  15. It takes two (centrioles) to tango.Reproduction (Cambridge, England) · 2019
    Review
  16. Article
  17. Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Paula A CoelhoCancer Research UK Cell Cycle Genetics Group, Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK; Pluripotency and Differentiation in Early Mouse Development Group, Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 1QN, UK.
Leah BuryCancer Research UK Cell Cycle Genetics Group, Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK; Pluripotency and Differentiation in Early Mouse Development Group, Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 1QN, UK.
Bedra SharifCancer Research UK Cell Cycle Genetics Group, Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK; Pluripotency and Differentiation in Early Mouse Development Group, Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 1QN, UK.
Maria G RiparbelliDepartment of Evolutionary Biology, University of Siena, Via A. Moro 41-53100, Siena, Italy.
Jingyan FuCancer Research UK Cell Cycle Genetics Group, Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK.
Giuliano CallainiDepartment of Evolutionary Biology, University of Siena, Via A. Moro 41-53100, Siena, Italy.
David M GloverCancer Research UK Cell Cycle Genetics Group, Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK. Electronic address: dmg25@mole.bio.cam.ac.uk.
Magdalena Zernicka-GoetzPluripotency and Differentiation in Early Mouse Development Group, Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 1QN, UK. Electronic address: mz205@cam.ac.uk.
Wellcome/Cancer Research UK Gurdon Institute · GBCancer Research UK · GBUniversity of Siena · IT

Funding

Cancer Research UK 11431Wellcome Trust 092096
6 · The paper itself

Abstract

During the first five rounds of cell division in the mouse embryo, spindles assemble in the absence of centrioles. Spindle formation initiates around chromosomes, but the microtubule nucleating process remains unclear. Here we demonstrate that Plk4, a protein kinase known as a master regulator of centriole formation, is also essential for spindle assembly in the absence of centrioles. Depletion of maternal Plk4 prevents nucleation and growth of microtubules and results in monopolar spindle formation. This leads to cytokinesis failure and, consequently, developmental arrest. We show that Plk4 function depends on its kinase activity and its partner protein, Cep152. Moreover, tethering Cep152 to cellular membranes sequesters Plk4 and is sufficient to trigger spindle assembly from ectopic membranous sites. Thus, the Plk4-Cep152 complex has an unexpected role in promoting microtubule nucleation in the vicinity of chromosomes to mediate bipolar spindle formation in the absence of centrioles.

Indexed as

AnimalsCell Cycle ProteinsCell DivisionCentriolesFemaleFetusMaleMeiosisMiceMice, Inbred C57BLMice, Inbred CBAMicrotubulesMitosisPregnancyProtein Serine-Threonine KinasesRNA, MessengerCell Cycle ProteinsPlk4 protein, mouseProtein Serine-Threonine KinasesRNA, Messenger

Identifiers

PMID24268700
PMCPMC3898710
OpenAlexW2163665905

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.