ArticleBiophysical journal2013
Viscoelasticity as a biomarker for high-throughput flow cytometry.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 34 citations in OpenAlex.
- Microfluidic technologies for cell deformability cytometry.Smart medicine · 2022Review
- A constriction channel analysis of astrocytoma stiffness and disease progression.Biomicrofluidics · 2021Article
- Modeling erythrocyte electrodeformation in response to amplitude modulated electric waveforms.Scientific reports · 2018Article
- High-throughput physical phenotyping of cell differentiation.Microsystems & nanoengineering · 2017Article
- A Comprehensive Review of Optical Stretcher for Cell Mechanical Characterization at Single-Cell Level.Micromachines · 2016Review
- High-throughput linear optical stretcher for mechanical characterization of blood cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2016Article
- Cellular enrichment through microfluidic fractionation based on cell biomechanical properties.Microfluidics and nanofluidics · 2015Article
- Imaging of a linear diode bar for an optical cell stretcher.Biomedical optics express · 2015Article
- Real-time deformability cytometry: on-the-fly cell mechanical phenotyping.Nature methods · 2015Article
- High-Throughput Assessment of Cellular Mechanical Properties.Annual review of biomedical engineering · 2015Review
- Characterization of mechanical and regenerative properties of human, adipose stromal cells.Cellular and molecular bioengineering · 2014Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
The mechanical properties of living cells are a label-free biophysical marker of cell viability and health; however, their use has been greatly limited by low measurement throughput. Although examining individual cells at high rates is now commonplace with fluorescence activated cell sorters, development of comparable techniques that nondestructively probe cell mechanics remains challenging. A fundamental hurdle is the signal response time. Where light scattering and fluorescence signatures are virtually instantaneous, the cell stress relaxation, typically occurring on the order of seconds, limits the potential speed of elastic property measurement. To overcome this intrinsic barrier to rapid analysis, we show here that cell viscoelastic properties measured at frequencies far higher than those associated with cell relaxation can be used as a means of identifying significant differences in cell phenotype. In these studies, we explore changes in erythrocyte mechanical properties caused by infection with Plasmodium falciparum and find that the elastic response alone fails to detect malaria at high frequencies. At timescales associated with rapid assays, however, we observe that the inelastic response shows significant changes and can be used as a reliable indicator of infection, establishing the dynamic viscoelasticity as a basis for nondestructive mechanical analogs of current high-throughput cell classification methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.