ArticleImmunobiology2014
Circulating complexes of the vitamin D binding protein with G-actin induce lung inflammation by targeting endothelial cells.
Article in Immunobiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Decrease in Vitamin D Binding Protein in Acute Fractures Does Not Affect Vitamin D Levels.Clinics in orthopedic surgery · 2025Article
- Vitamin D and vitamin D binding protein levels in COVID-19 intensive care unit patients: A prospective multicenter study.Journal of medical biochemistry · 2024Article
- Vitamin D binding protein: A polymorphic protein with actin-binding capacity in COVID-19.Nutrition (Burbank, Los Angeles County, Calif.) · 2022Article
- Very Low Vitamin D Levels are a Strong Independent Predictor of Mortality in Hospitalized Patients with Severe COVID-19.Archives of medical research · 2022Article
- Endothelial injury, F-actin and vitamin-D binding protein after hematopoietic stem cell transplant and association with clinical outcomes.Haematologica · 2021Article
- Vitamin D binding protein: A key regulator of vitamin D deficiency among patients with pneumonia.Clinical nutrition (Edinburgh, Scotland) · 2021Article
- Genetic Polymorphisms in the Host and COVID-19 Infection.Advances in experimental medicine and biology · 2021Article
- Potential role of GcMAF in suppressing the severity of COVID-19-induced immune responses: Lesson learned from HIV.Medical hypotheses · 2020Article
- Vitamin D binding protein in COVID-19.Clinical medicine (London, England) · 2020Article
- Association between low vitamin D and COVID-19: don't forget the vitamin D binding protein.Aging clinical and experimental research · 2020Article
- Maternal vitamin D deficiency impairs Treg and Breg responses in offspring mice and deteriorates allergic airway inflammation.Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology · 2020Article
- Modifiers of Plasma 25-Hydroxyvitamin D and Chronic Kidney Disease Outcomes in Black Americans: The Jackson Heart Study.The Journal of clinical endocrinology and metabolism · 2019Article
- Vitamin D Binding Protein: A Historic Overview.Frontiers in endocrinology · 2019Review
- The Vitamin D Binding Protein and Inflammatory Injury: A Mediator or Sentinel of Tissue Damage?Frontiers in endocrinology · 2019Review
- Vitamin D-binding protein deficiency in mice decreases systemic and select tissue levels of inflammatory cytokines in a murine model of acute muscle injury.The journal of trauma and acute care surgery · 2018Article
- Site-specific and endothelial-mediated dysfunction of the alveolar-capillary barrier in response to lipopolysaccharides.Journal of cellular and molecular medicine · 2018Article
- Membrane array and multiplex bead analysis of tear cytokines in systemic sclerosis.Immunologic research · 2016Article
- Vitamin D binding protein and vitamin D in human allergen-induced endobronchial inflammation.Clinical and experimental immunology · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
This study investigated the actin scavenger function of the vitamin D binding protein (DBP) in vivo using DBP null (-/-) mice. Intravenous injection of G-actin into wild-type (DBP+/+) and DBP-/- mice showed that contrary to expectations, DBP+/+ mice developed more severe acute lung inflammation. Inflammation was restricted to the lung and pathological changes were clearly evident at 1.5 and 4h post-injection but were largely resolved by 24h. Histology of DBP+/+ lungs revealed noticeably more vascular leakage, hemorrhage and thickening of the alveolar wall. Flow cytometry analysis of whole lung homogenates showed significantly increased neutrophil infiltration into DBP+/+ mouse lungs at 1.5 and 4h. Increased amounts of protein and leukocytes were also noted in bronchoalveolar lavage fluid from DBP+/+ mice 4h after actin injection. In vitro, purified DBP-actin complexes did not activate complement or neutrophils but induced injury and death of cultured human lung microvascular endothelial cells (HLMVEC) and human umbilical vein endothelial cells (HUVEC). Cells treated with DBP-actin showed a significant reduction in viability at 4h, this effect was reversible if cells were cultured in fresh media for another 24h. However, a 24-h treatment with DBP-actin complexes showed a significant increase in cell death (95% for HLMVEC, 45% for HUVEC). The mechanism of endothelial cell death was via both caspase-3 dependent (HUVEC) and independent (HLMVEC) pathways. These results demonstrate that elevated levels and/or prolonged exposure to DBP-actin complexes may induce endothelial cell injury and death, particularly in the lung microvasculature.
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Registered trials
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