ArticleJournal of neurosurgery2014
Overexpression of adenosine kinase in cortical astrocytes and focal neocortical epilepsy in mice.
Article in Journal of neurosurgery, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 35 citations in OpenAlex.
- Calponin-3 is associated with epilepsy through the regulation of astrocyte activity.Brain pathology (Zurich, Switzerland) · 2026Article
- Research progress in glioma-related epilepsy (Review).Biomedical reports · 2025Article
- Adenosine Kinase: An Epigenetic Modulator and Drug Target.Journal of inherited metabolic disease · 2025Review
- Neuroglia and brain energy metabolism.Handbook of clinical neurology · 2025Review
- Overview Article Astrocytes as Initiators of Epilepsy.Neurochemical research · 2023Review
- Review
- Adeno-Associated Viruses for Modeling Neurological Diseases in Animals: Achievements and Prospects.Biomedicines · 2022Review
- Adenosine-AFrontiers in pharmacology · 2022Article
- Adenosine kinase: A key regulator of purinergic physiology.Biochemical pharmacology · 2021Review
- Downregulation of Retinal Connexin 43 in GFAP-Expressing Cells Modifies Vasoreactivity Induced by Perfusion Ocular Pressure Changes.Investigative ophthalmology & visual science · 2021Article
- MicroRNAs as regulators of brain function and targets for treatment of epilepsy.Nature reviews. Neurology · 2020Review
- Aberrant energy metabolism and redox balance in seizure onset zones of epileptic patients.Journal of proteomics · 2020Article
- Brain somatic mutations in MTOR reveal translational dysregulations underlying intractable focal epilepsy.The Journal of clinical investigation · 2019Article
- The role of adenosine in epilepsy.Brain research bulletin · 2019Review
- Increased expression of the P2X7 receptor in temporal lobe epilepsy: Animal models and clinical evidence.Molecular medicine reports · 2019Article
- Dynamic Changes of Astrocytes and Adenosine Signaling in Rat Hippocampus in Post-status Epilepticus Model of Epileptogenesis.Cellular and molecular neurobiology · 2018Article
- Epilepsy and astrocyte energy metabolism.Glia · 2018Review
- Astroglial CaFrontiers in cellular neuroscience · 2018Article
- Validation of a Preclinical Drug Screening Platform for Pharmacoresistant Epilepsy.Neurochemical research · 2017Article
- Adenosine Kinase Deficiency in the Brain Results in Maladaptive Synaptic Plasticity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
objectNew experimental models and diagnostic methods are needed to better understand the pathophysiology of focal neocortical epilepsies in a search for improved epilepsy treatment options. The authors hypothesized that a focal disruption of adenosine homeostasis in the neocortex might be sufficient to trigger electrographic seizures. They further hypothesized that a focal disruption of adenosine homeostasis might affect microcirculation and thus offer a diagnostic opportunity for the detection of a seizure focus located in the neocortex.
methodsFocal disruption of adenosine homeostasis was achieved by injecting an adeno-associated virus (AAV) engineered to overexpress adenosine kinase (ADK), the major metabolic clearance enzyme for the brain's endogenous anticonvulsant adenosine, into the neocortex of mice. Eight weeks following virus injection, the affected brain area was imaged via optical microangiography (OMAG) to detect changes in microcirculation. After completion of imaging, cortical electroencephalography (EEG) recordings were obtained from the imaged brain area.
resultsViral expression of the Adk cDNA in astrocytes generated a focal area (~ 2 mm in diameter) of ADK overexpression within the neocortex. OMAG scanning revealed a reduction in vessel density within the affected brain area of approximately 23% and 29% compared with control animals and the contralateral hemisphere, respectively. EEG recordings revealed electrographic seizures within the focal area of ADK overexpression at a rate of 1.3 ± 0.2 seizures per hour (mean ± SEM).
conclusionsThe findings of this study suggest that focal adenosine deficiency is sufficient to generate a neocortical focus of hyperexcitability, which is also characterized by reduced vessel density. The authors conclude that their model constitutes a useful tool to study neocortical epilepsies and that OMAG constitutes a noninvasive diagnostic tool for the imaging of seizure foci with disrupted adenosine homeostasis.
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Registered trials
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