Trial reportTissue engineering. Part C, Methods2014
A human in vitro model that mimics the renal proximal tubule.
Trial report in Tissue engineering. Part C, Methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Isolation and functional characterization of primary human proximal tubular epithelial cells from brain-dead organ donors.Physiological reports · 2026Article
- Sustained alterations in proximal tubule gene expression in primary culture associate with HNF4A loss.Scientific reports · 2024Article
- Efficient proximal tubule-on-chip model from hiPSC-derived kidney organoids for functional analysis of renal transporters.iScience · 2024Article
- Patient-Derived Lung Cancer "Sandwich Cultures" with a Preserved Tumor Microenvironment.Tissue engineering. Part C, Methods · 2024Article
- Expired Platelet Concentrate Up-Cycling: Growth Factor-Rich Bioproduct Preparation for FBS Substitute.Journal of clinical medicine · 2023Article
- User-friendly microfluidic system reveals native-like morphological and transcriptomic phenotypes induced by shear stress in proximal tubule epithelium.APL bioengineering · 2023Article
- Optimization of Primary Human Bronchial Epithelial 3D Cell Culture with Donor-Matched Fibroblasts and Comparison of Two Different Culture Media.International journal of molecular sciences · 2023Article
- Inhibition of Transforming Growth Factor-β Improves Primary Renal Tubule Cell Differentiation in Long-Term Culture.Tissue engineering. Part A · 2023Article
- Functional Drug Screening using Kidney Cells On-A-Chip: Advances in Disease Modeling and Development of Biomarkers.Kidney360 · 2022Article
- Microphysiological systems in absorption, distribution, metabolism, and elimination sciences.Clinical and translational science · 2022Review
- Application of Porcine Kidney-Derived Extracellular Matrix as Coating, Hydrogel, and Scaffold Material for Renal Proximal Tubular Epithelial Cell.BioMed research international · 2022Article
- Modeling the Glomerular Filtration Barrier and Intercellular Crosstalk.Frontiers in physiology · 2021Review
- Renal proximal tubular epithelial cells: review of isolation, characterization, and culturing techniques.Molecular biology reports · 2020Review
- ROR1-CAR T cells are effective against lung and breast cancer in advanced microphysiologic 3D tumor models.JCI insight · 2019Article
- A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier.Nature communications · 2019Article
- Substrate Elasticity Governs Differentiation of Renal Tubule Cells in Prolonged Culture.Tissue engineering. Part A · 2019Article
- Article
- Liver and Kidney on Chips: Microphysiological Models to Understand Transporter Function.Clinical pharmacology and therapeutics · 2016Review
- Mimicking Metastases Including Tumor Stroma: A New Technique to Generate a Three-Dimensional Colorectal Cancer Model Based on a Biological Decellularized Intestinal Scaffold.Tissue engineering. Part C, Methods · 2016Article
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human in vitro-manufactured tissue and organ models can serve as powerful enabling tools for the exploration of fundamental questions regarding cell, matrix, and developmental biology in addition to the study of drug delivery dynamics and kinetics. To date, the development of a human model of the renal proximal tubule (PT) has been hindered by the lack of an appropriate cell source and scaffolds that allow epithelial monolayer formation and maintenance. Using extracellular matrices or matrix proteins, an in vivo-mimicking environment can be created that allows epithelial cells to exhibit their typical phenotype and functionality. Here, we describe an in vitro-engineered PT model. We isolated highly proliferative cells from cadaveric human kidneys (human kidney-derived cells [hKDCs]), which express markers that are associated with renal progenitor cells. Seeded on small intestinal submucosa (SIS), hKDCs formed a confluent monolayer and displayed the typical phenotype of PT epithelial cells. PT markers, including N-cadherin, were detected throughout the hKDC culture on the SIS, whereas markers of later tubule segments were weak (E-cadherin) or not (aquaporin-2) expressed. Basement membrane and microvilli formation demonstrated a strong polarization. We conclude that the combination of hKDCs and SIS is a suitable cell-scaffold composite to mimic the human PT in vitro.
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