ArticleEuropean journal of microbiology & immunology2013
Extracellular secretion of protease HtrA from Campylobacter jejuni is highly efficient and independent of its protease activity and flagellum.
Article in European journal of microbiology & immunology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 27 citations in OpenAlex.
- Protease-serpin-microbiome interactions in inflammatory bowel disease: toward integrated biomarkers of gut health.Gut microbes · 2026Review
- Impact of Isoquinoline Alkaloids on the Intestinal Barrier in a Colonic Model ofInternational journal of molecular sciences · 2025Article
- Pathogenicity and virulence ofVirulence · 2024Review
- Identification and Characterization of an HtrA Sheddase Produced byInternational journal of molecular sciences · 2023Article
- Evolution and Role of Proteases inBiomolecules · 2023Review
- Gut Serpinome: Emerging Evidence in IBD.International journal of molecular sciences · 2021Review
- In Silico Structural and Functional Characterization of HtrA Proteins ofTropical medicine and infectious disease · 2020Article
- Functional analysis and cryo-electron microscopy ofGut microbes · 2020Article
- Extracellular Pneumococcal Serine Proteases Affect Nasopharyngeal Colonization.Frontiers in cellular and infection microbiology · 2020Article
- Protease Activity ofFrontiers in cellular and infection microbiology · 2019Article
- Quantification of serine protease HtrA molecules secreted by the foodborne pathogenGut pathogens · 2019Article
- Function of Serine Protease HtrA in the Lifecycle of the Foodborne PathogenEuropean journal of microbiology & immunology · 2018Review
- Amino-Terminal Processing ofFrontiers in microbiology · 2018Article
- Bacterial serine protease HtrA as a promising new target for antimicrobial therapy?Cell communication and signaling : CCS · 2017Article
- HtrA-mediated E-cadherin cleavage is limited to DegP and DegQ homologs expressed by gram-negative pathogens.Cell communication and signaling : CCS · 2016Article
- Characterisation of worldwide Helicobacter pylori strains reveals genetic conservation and essentiality of serine protease HtrA.Molecular microbiology · 2016Article
- Campylobacter jejuni serine protease HtrA plays an important role in heat tolerance, oxygen resistance, host cell adhesion, invasion, and transmigration.European journal of microbiology & immunology · 2015Article
- The role of serine protease HtrA in acute ulcerative enterocolitis and extra-intestinal immune responses during Campylobacter jejuni infection of gnotobiotic IL-10 deficient mice.Frontiers in cellular and infection microbiology · 2014Article
- The impact of serine protease HtrA in apoptosis, intestinal immune responses and extra-intestinal histopathology during Campylobacter jejuni infection of infant mice.Gut pathogens · 2014Article
- Transmigration route of Campylobacter jejuni across polarized intestinal epithelial cells: paracellular, transcellular or both?Cell communication and signaling : CCS · 2013Review
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
Abstract
The serine protease HtrA of C. jejuni has been identified as a novel secreted virulence factor which opens cell-to-cell junctions by cleaving E-cadherin. Efficient C. jejuni transmigration across polarized human epithelial cells requires the intact flagellum and HtrA; however, the mechanism of HtrA secretion into the supernatant is unknown. Here we show that HtrA secretion is highly efficient and does not require its proteolytic activity because the protease-inactive S197A mutant is secreted like wild-type HtrA. In addition, the flagellar mutants ΔflaA/B, ΔfliI, ΔflgH, ΔflhA, ΔflhB, and ΔflgS were also able to secrete HtrA in high amounts, while they were strongly attenuated in secreting the well-known invasion antigen CiaB. We also tested several culture media and cell lines of different origin such as human, mouse, hamster, dog, and chicken for their ability to influence HtrA secretion. Interestingly, HtrA was effectively secreted in the presence of most but not all cell lines and media, albeit at different levels, but secretion was significantly higher when fetal calf serum (FCS) was added. These results demonstrate that HtrA secretion by Campylobacter proceeds independent of HtrA's protease activity, the flagellum and origin of cell lines, but can be strongly enhanced by molecular compound(s) present in FCS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.