ArticleEuropean journal of microbiology & immunology2013
Diagnostic performance and therapeutic impact of LightCycler SeptiFast assay in patients with suspected sepsis.
Article in European journal of microbiology & immunology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Accuracy of LightCycler(®) SeptiFast for the detection and identification of pathogens in the blood of patients with suspected sepsis: a systematic review and meta-analysis.Intensive care medicine · 2015Pooled it
- Rapid molecular assays versus blood culture for bloodstream infections: a systematic review and meta-analysis.EClinicalMedicine · 2025Article
- Performance of Multiplex PCR and β-1,3-D-Glucan Testing for the Diagnosis of Candidemia.Journal of fungi (Basel, Switzerland) · 2022Article
- The Clinical Impact of Rapid Molecular Microbiological Diagnostics for Pathogen and Resistance Gene Identification in Patients With Sepsis: A Systematic Review.Open forum infectious diseases · 2020Review
- The challenge of molecular diagnosis of bloodstream infections.World journal of microbiology & biotechnology · 2019Review
- Emerging Technologies for Molecular Diagnosis of Sepsis.Clinical microbiology reviews · 2018Review
- SeptiFast versus blood culture in clinical routine - A report on 3 years experience.Wiener klinische Wochenschrift · 2017Observational
- The new frontier of diagnostics: Molecular assays and their role in infection prevention and control.American journal of infection control · 2017Review
- Development of a new real-time PCR system for simultaneous detection of bacteria and fungi in pathological samples.International journal of clinical and experimental pathology · 2015Article
- Emerging technologies for rapid identification of bloodstream pathogens.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2014Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid and reliable identification of pathogens is very important in the management of septic patients. We retrospectively evaluated the diagnostic accuracy and clinical utility of a multiplex real-time polymerase chain reaction (PCR) assay (SeptiFast (SF)) in patients with suspected sepsis in a tertiary care hospital in Tallinn, Estonia. A total of 160 blood samples from 144 patients were included in the study. SF results were compared with corresponding blood culture (BC) results. The concordance between SF and BC was 78.8%. The rate of positive results was significantly higher in SF than in BC (33.7% vs. 21.2%, respectively; p < 0.001). A total of 27 samples were found positive by both SF and BC, 27 by SF only, and seven by BC only. Of a total of 83 microorganisms detected SF identified 71, and BC 42 (p < 0.001). SF detected markedly more patients with candidemia: 11 patients were detected by SF compared to four patients by BC. Antimicrobial treatment was changed in 21 (38.9%) of 54 SF positive cases. In conclusion, our results demonstrated the high diagnostic accuracy of SF in detection of sepsis pathogens. In conjunction with its impact on therapeutic decisions, SF proved to be a useful adjunct to conventional blood culture in the diagnosis of sepsis etiology.
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