Evidence map›Paper›PMID 24265813›Full record

ArticlePloS one2013

Transcriptome analysis of leaf tissue of Raphanus sativus by RNA sequencing.

Libin Zhang, Haibo Jia, Yongtai Yin, Gang Wu, Heng Xia, Xiaodong Wang, Chunhua Fu, Maoteng Li, Jiangsheng Wu

Abstract read
In one paragraph

Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Libin ZhangSchool of Life Science and Techonology, Huazhong University of Science and Technology, Wuhan, Hubei, China ; Institute of Biology and Medicine, Wuhan University of Science and Technology, Wuhan, Hubei, China.
Haibo Jia
Yongtai Yin
Gang Wu
Heng Xia
Xiaodong Wang
Chunhua Fu
Maoteng Li
Jiangsheng Wu

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Raphanus sativus is not only a popular edible vegetable but also an important source of medicinal compounds. However, the paucity of knowledge about the transcriptome of R. sativus greatly impedes better understanding of the functional genomics and medicinal potential of R. sativus. In this study, the transcriptome sequencing of leaf tissues in R. sativus was performed for the first time. Approximately 22 million clean reads were generated and used for transcriptome assembly. The generated unigenes were subsequently annotated against gene ontology (GO) database. KEGG analysis further revealed two important pathways in the bolting stage of R.sativus including spliceosome assembly and alkaloid synthesis. In addition, a total of 6,295 simple sequence repeats (SSRs) with various motifs were identified in the unigene library of R. sativus. Finally, four unigenes of R. sativus were selected for alignment with their homologs from other plants, and phylogenetic trees for each of the genes were constructed. Taken together, this study will provide a platform to facilitate gene discovery and advance functional genomic research of R. sativus.

Indexed as

Plant LeavesTranscriptomeComputational BiologyGene Expression ProfilingGene Regulatory NetworksMicrosatellite RepeatsMolecular Sequence AnnotationPhylogenyRaphanusSequence Analysis, RNASpecies Specificity

Identifiers

PMID24265813
PMCPMC3827192

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.