ArticlePloS one2013
The high-affinity phosphodiesterase BcPde2 has impact on growth, differentiation and virulence of the phytopathogenic ascomycete Botrytis cinerea.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 25 citations in OpenAlex.
- Challenges and Opportunities Arising from Host-International journal of molecular sciences · 2024Review
- Structural diversification of fungal cell wall in response to the stress signaling and remodeling during fungal pathogenesis.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- A cAMP phosphodiesterase is essential for sclerotia formation and virulence inFrontiers in plant science · 2023Article
- Functional Analysis of Two Affinity cAMP Phosphodiesterases in the Nematode-Trapping FungusPathogens (Basel, Switzerland) · 2022Article
- The NecrotrophFrontiers in plant science · 2022Article
- Article
- The Destructive Fungal PathogenPathogens (Basel, Switzerland) · 2020Review
- Article
- Caffeine fostering of mycoparasitic fungi against phytopathogens.Plant signaling & behavior · 2016Article
- Developmental Growth Control Exerted via the Protein A Kinase Tpk2 in Ashbya gossypii.Eukaryotic cell · 2015Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Components of the cAMP signaling pathway, such as the adenylate cyclase Bac and the protein kinase A (PKA) were shown to affect growth, morphogenesis and differentiation as well as virulence of the phytopathogenic fungus Botrytis cinerea. While loss of Bac caused drastically reduced intracellular cAMP levels, deletion of the PKA resulted in extremely increased cAMP concentrations. To regulate the intracellular level of the second messenger cAMP, a balance between its biosynthesis through adenylate cyclase activity and its hydrolysis by phosphodiesterases (PDEs) is crucial. Here, we report the functional characterization of the two PDEs in the ascomycete B. cinerea, BcPde1 and BcPde2. While deletion of bcpde2 resulted in severely affected vegetative growth, conidiation, germination and virulence, the bcpde1 deletion strain displayed a wild-type-like phenotype. However, the double bcpde1/2 deletion mutant exhibited an even stronger phenotype. Localization studies revealed that BcPde2 accumulates at the plasma membrane, but is also localized in the cytoplasm. BcPde1 was shown to be distributed in the cytoplasm as well, but also accumulates in so far unknown mobile vesicles. Overexpression of bcpde1 in the Δbcpde2 background rescued the deletion phenotype, and in addition an increased transcript level of bcpde1 in the Δbcpde2 strain was observed, indicating redundant functions of both PDEs and an interdependent gene expression.
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