ArticleThe Journal of biological chemistry2013
Protein kinase D promotes airway epithelial barrier dysfunction and permeability through down-regulation of claudin-1.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 31 citations in OpenAlex.
- Epithelial cells in chronic obstructive pulmonary disease exacerbations: Targets for lung repair.Pharmacological reviews · 2026Review
- Synergistic Enhancement of Isoforskolin and Dexamethasone Against Sepsis and Acute Lung Injury Mouse Models.Journal of inflammation research · 2023Article
- Perfluorooctane Sulfonic Acid Disrupts Protective Tight Junction Proteins via Protein Kinase D in Airway Epithelial Cells.Toxicological sciences : an official journal of the Society of Toxicology · 2022Article
- Effects of mucopolysaccharide polysulphate on tight junction barrier in human epidermal keratinocytes.Experimental dermatology · 2022Article
- A Single-Cell Atlas of Large and Small Airways at Birth in a Porcine Model of Cystic Fibrosis.American journal of respiratory cell and molecular biology · 2022Article
- Review
- Increased integrity of cell-cell junctions accompanied by increased expression of claudin 4 in keratinocytes stimulated with vitamin D3.Medical molecular morphology · 2021Article
- The Epithelial Cell Leak Pathway.International journal of molecular sciences · 2021Review
- Airway tight junctions as targets of viral infections.Tissue barriers · 2021Article
- Inhibiting Protein Kinase D Promotes Airway Epithelial Barrier Integrity in Mouse Models of Influenza A Virus Infection.Frontiers in immunology · 2020Article
- Interleukin-13 Alters Tight Junction Proteins Expression Thereby Compromising Barrier Function and Dampens Rhinovirus Induced Immune Responses in Nasal Epithelium.Frontiers in cell and developmental biology · 2020Article
- ADAM15 mediates upregulation of Claudin-1 expression in breast cancer cells.Scientific reports · 2019Article
- Trophoblast cell surface antigen 2 (Trop-2) phosphorylation by protein kinase C α/δ (PKCα/δ) enhances cell motility.The Journal of biological chemistry · 2019Article
- Disruption of the airway epithelial barrier in a murine model of respiratory syncytial virus infection.American journal of physiology. Lung cellular and molecular physiology · 2019Article
- The possible association between AQP9 in the intestinal epithelium and acute liver injury‑induced intestinal epithelium damage.Molecular medicine reports · 2018Article
- Monocytic cell junction proteins serve important roles in atherosclerosis via the endoglin pathway.Molecular medicine reports · 2017Article
- Airborne Particulate Matter Induces Nonallergic Eosinophilic Sinonasal Inflammation in Mice.American journal of respiratory cell and molecular biology · 2017Article
- Cytokine-Ion Channel Interactions in Pulmonary Inflammation.Frontiers in immunology · 2017Review
- Maresin 1 Maintains the Permeability of Lung Epithelial Cells In Vitro and In Vivo.Inflammation · 2016Article
- Protein Kinase D2 Protects against Acute Colitis Induced by Dextran Sulfate Sodium in Mice.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
At the interface between host and external environment, the airway epithelium serves as a major protective barrier. In the present study we show that protein kinase D (PKD) plays an important role in the formation and integrity of the airway epithelial barrier. Either inhibition of PKD activity or silencing of PKD increased transepithelial electrical resistance (TEER), resulting in a tighter epithelial barrier. Among the three PKD isoforms, PKD3 knockdown was the most efficient one to increase TEER in polarized airway epithelial monolayers. In contrast, overexpression of PKD3 wild type, but not PKD3 kinase-inactive mutant, disrupted the formation of apical intercellular junctions and their reassembly, impaired the development of TEER, and increased paracellular permeability to sodium fluorescein in airway epithelial monolayers. We further found that overexpression of PKD, in particular PKD3, markedly suppressed the mRNA and protein levels of claudin-1 but had only minor effects on the expression of other tight junctional proteins (claudin-3, claudin-4, claudin-5, occludin, and ZO-1) and adherent junctional proteins (E-cadherin and β-catenin). Immunofluorescence study revealed that claudin-1 level was markedly reduced and almost disappeared from intercellular contacts in PKD3-overexpressed epithelial monolayers and that claudin-4 was also restricted from intercellular contacts and tended to accumulate in the cell cytosolic compartments. Last, we found that claudin-1 knockdown prevented TEER elevation by PKD inhibition or silencing in airway epithelial monolayers. These novel findings indicate that PKD negatively regulates human airway epithelial barrier formation and integrity through down-regulation of claudin-1, which is a key component of tight junctions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.