ArticleNucleic acids research2014
Method for site-specific detection of m6A nucleoside presence in RNA based on high-resolution melting (HRM) analysis.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed.
- Therapy-induced mRNA, rRNA and tRNA methylation alterations confer tolerance phenotype in tumor cells: mechanism and implications.International journal of biological sciences · 2026Review
- When animal viruses meet NVeterinary research · 2024Review
- An Overview of Current Detection Methods for RNA Methylation.International journal of molecular sciences · 2024Review
- The emerging roles of N6-methyladenosine (m6A)-modified long non-coding RNAs in human cancers.Cell death discovery · 2022Review
- Research progress concerning mOncology letters · 2021Review
- RNA NJournal of immunology research · 2020Review
- Function and evolution of RNA N6-methyladenosine modification.International journal of biological sciences · 2020Review
- Multifaceted Functions and Novel Insight Into the Regulatory Role of RNA NFrontiers in cell and developmental biology · 2020Review
- Screening of differential expressed genes from gene chip and sequencing data and evaluate its prognostic values in males with digestive system neoplasms.Translational cancer research · 2019Article
- Regulation of Viral Infection by the RNA ModificationAnnual review of virology · 2019Review
- Significant epitranscriptomes in heterogeneous cancer.Cancer science · 2019Review
- Detection of N6‑methyladenosine modification residues (Review).International journal of molecular medicine · 2019Review
- Functions of RNA N6-methyladenosine modification in cancer progression.Molecular biology reports · 2019Review
- Functions of RNA N6-methyladenosine modification in cancer progression.Molecular biology reports · 2019Review
- Emerging approaches for detection of methylation sites in RNA.Open biology · 2018Review
- RNA Methylation in ncRNA: Classes, Detection, and Molecular Associations.Frontiers in genetics · 2018Article
- A Method for Measuring RNA N 6-methyladenosine Modifications in Cells and Tissues.Journal of visualized experiments : JoVE · 2016Article
- Article
- Posttranscriptional modification of messenger RNAs in eukaryotes.Molecular biology · 2015Review
- Experimental and Computational Tools for Mapping Circular RNA Modifications.Wiley interdisciplinary reviews. RNAReview
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Chemical landscape of natural RNA species is decorated with the large number of modified nucleosides. Some of those could easily be detected by reverse transcription, while others permit only high-performance liquid chromatography or mass-spectrometry detection. Presence of m(6)A nucleoside at a particular position of long RNA molecule is challenging to observe. Here we report an easy and high-throughput method for detection of m(6)A nucleosides in RNA based on high-resolution melting analysis. The method relies on the previous knowledge of the modified nucleoside position at a particular place of RNA and allows rapid screening for conditions or genes necessary for formation of that modification.
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