ArticleNature protocols2013
Generating suspended cell monolayers for mechanobiological studies.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 71 citations in OpenAlex.
- A biohybrid system to reconstruct epithelial morphomechanicsMaterials today. Bio · 2026Article
- Tissue tension of planar, free standing cell monolayers measured by central deformation.PNAS nexus · 2025Article
- Mechanical properties of adherent cell sheets analyzed by deflection of supporting membrane.European biophysics journal : EBJ · 2025Article
- The TissueTractor: A Device for Applying Large Strains to Tissues and Cells for Simultaneous High-Resolution Live Cell Microscopy.Small methods · 2025Article
- Article
- Origin of yield stress and mechanical plasticity in model biological tissues.Nature communications · 2025Article
- Rupture strength of living cell monolayers.Nature materials · 2024Article
- The TissueTractor, a device for applying large strains to tissues and cells for simultaneous high-resolution live cell microscopy.bioRxiv : the preprint server for biology · 2024Article
- Active viscoelastic models for cell and tissue mechanics.Royal Society open science · 2024Article
- Engineering tools for quantifying and manipulating forces in epithelia.Biophysics reviews · 2023Review
- A magnetically actuated, optically sensed tensile testing method for mechanical characterization of soft biological tissues.Science advances · 2023Article
- Tension at intercellular junctions is necessary for accurate orientation of cell division in the epithelium plane.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Mechanical properties of cell sheets and spheroids: the link between single cells and complex tissues.Biophysical reviews · 2021Review
- To form and function: on the role of basement membrane mechanics in tissue development, homeostasis and disease.Open biology · 2021Review
- Measuring mechanical stress in living tissues.Nature reviews. Physics · 2020Article
- Curling of epithelial monolayers reveals coupling between active bending and tissue tension.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Measurement of junctional tension in epithelial cells at the onset of primitive streak formation in the chick embryo via non-destructive optical manipulation.Development (Cambridge, England) · 2020Article
- Actomyosin controls planarity and folding of epithelia in response to compression.Nature materials · 2020Article
- A unified rheological model for cells and cellularised materials.Royal Society open science · 2020Article
- Stress relaxation in epithelial monolayers is controlled by the actomyosin cortex.Nature physics · 2019Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
Abstract
Cell monolayers line most of the surfaces and cavities in the human body. During development and normal physiology, monolayers sustain, detect and generate mechanical stresses, yet little is known about their mechanical properties. We describe a cell culture and mechanical testing protocol for generating freely suspended cell monolayers and examining their mechanical and biological response to uniaxial stretch. Cells are cultured on temporary collagen scaffolds polymerized between two parallel glass capillaries. Once cells form a monolayer covering the collagen and the capillaries, the scaffold is removed with collagenase, leaving the monolayer suspended between the test rods. The suspended monolayers are subjected to stretching by prying the capillaries apart with a micromanipulator. The applied force can be measured for the characterization of monolayer mechanics. Monolayers can be imaged with standard optical microscopy to examine changes in cell morphology and subcellular organization concomitant with stretch. The entire preparation and testing protocol requires 3-4 d.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.