ArticleAmerican journal of obstetrics and gynecology2014
Vitamin D regulates contractile profile in human uterine myometrial cells via NF-κB pathway.
Article in American journal of obstetrics and gynecology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 2 syntheses or guidelines pooled it, 49 citations in OpenAlex.
- Vitamin D Supplementation for Patients with Dysmenorrhoea: A Meta-Analysis with Trial Sequential Analysis of Randomised Controlled Trials.Nutrients · 2024Pooled it
- The effects of vitamin D and calcium on primary dysmenorrhea: a systematic review.Journal of medicine and life · 2023Pooled it
- Effect of vitamin D and E supplementation on pain relief and premenstrual symptoms in primary dysmenorrhea: a randomized controlled trial.BMC women's health · 2025Trial
- Mode of delivery and maternal vitamin D deficiency: an optimized intelligent Bayesian network algorithm analysis of a stratified randomized controlled field trial.Scientific reports · 2023Trial
- Self-Reported Changes in Premenstrual and Menstrual Symptoms among Females Taking a Novel Multinutrient Supplement: A Pilot Study.Current developments in nutrition · 2026Article
- Review
- Review
- Article
- Complementary and alternative therapies in the treatment of primary dysmenorrhea.Frontiers in reproductive health · 2025Review
- Predictive value of Vitamin D levels in pregnant women on gestational length and neonatal weight in China: a population-based retrospective study.Reproductive biology and endocrinology : RB&E · 2024Article
- The association between maternal vitamin D deficiency and postpartum hemorrhage and uterine atony.Acta obstetricia et gynecologica Scandinavica · 2024Article
- The effect of vitamin D on adolescents' primary dysmenorrhea.Journal of medicine and life · 2023Article
- Vitamin D and vitamin D receptor polymorphism in Asian adolescents with primary dysmenorrhea.BMC women's health · 2023Article
- Article
- Vitamin Effects in Primary Dysmenorrhea.Life (Basel, Switzerland) · 2023Review
- Severity of Menstrual Pain Is Associated with Nutritional Intake and Lifestyle Habits.Healthcare (Basel, Switzerland) · 2023Article
- Low Maternal Serum 25-Hydroxyvitamin D Concentration Is Associated With Postpartum Hemorrhage: A Retrospective Observational Study.Frontiers in endocrinology · 2022Observational
- Vitamin D supplementation for primary dysmenorrhea: a double-blind, randomized, placebo-controlled trial.Obstetrics & gynecology science · 2021Article
- Review
- Vitamin D Supplementation Improves Uterine Receptivity in a Rat Model of Vitamin D Deficiency: A Possible Role of HOXA-10/FKBP52 Axis.Frontiers in physiology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveInfection triggers inflammation that, in turn, enhances the expression of contractile-associated factors in myometrium and increases the risk of preterm delivery. In this study, we assessed vitamin D regulation of inflammatory markers, contractile-associated factors, steroid hormone receptors, and NFκB pathway proteins in human uterine myometrial smooth muscle (UtSM) cells that were cultured in an inflammatory environment. STUDY
designInflammatory environment was simulated for UtSM cells by coculturing them with monocyte lineage (THP1) cells. We measured the expression of inflammatory markers, contractile-associated factors, steroid hormone receptors, and NFκB pathway proteins in UtSM cells that were cultured with THP1 cells in the presence and absence of vitamin D by real time polymerase chain reaction and Western blot analysis.
resultsMonocytes secreted monocyte inflammatory protein-1α and -1β, interleukin (IL)-1β and 6, and tumor necrosis factor-α into the conditioned medium. In the UtSM cells that had been cocultured with THP1 cells, there was a significant (P < .05) increase in the expression of inflammatory markers IL-1β, -6, and -13 and tumor necrosis factor-α; the contractile-associated factors connexin-43, Cox-2, and prostaglandin F2α receptor; the estrogen receptor α, and progesterone receptors A and B. Vitamin D treatment of cocultures decreased (P < .05) the expression of inflammatory markers and contractile-associated factors in UtSM cells. Similarly, vitamin D decreased estrogen receptor α and progesterone receptors A-to-B ratio in UtSM cells that were cocultured with THP1 cells. In addition, vitamin D treatment significantly (P < .05) decreased monocyte-induced p-IκBα in cytosol and NFκB-p65 in the nucleus and increased IκBα in cytosol in UtSM cells.
conclusionOur results suggest that vitamin D treatment decreases inflammation-induced cytokines and contractile-associated factors in the uterine myometrial smooth muscle cells through the NFκB pathway.
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Registered trials
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