ArticleBMC evolutionary biology2013
Reconstruction of the ancestral marsupial karyotype from comparative gene maps.
Article in BMC evolutionary biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 1 citations in OpenAlex.
- Leveraging Synteny to Generate Reference Genomes for Conservation: Assembling the Genomes of Hector's and Māui Dolphins.Molecular ecology resources · 2025Article
- Development of the pulmonary vasculature in the gray short-tailed opossum (Monodelphis domestica)-3D reconstruction by microcomputed tomography.Anatomical record (Hoboken, N.J. : 2007) · 2025Article
- Chromosome-level echidna genome illuminates evolution of multiple sex chromosome system in monotremes.GigaScience · 2025Article
- Compensation of gene dosage on the mammalian X.Development (Cambridge, England) · 2024Review
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- 3D reconstruction of the bronchial tree of the Gray short-tailed opossum (Monodelphis domestica) in the postnatal period.Journal of anatomy · 2023Article
- Evolution of the ancestral mammalian karyotype and syntenic regions.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Microchromosomes are building blocks of bird, reptile, and mammal chromosomes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- During evolution from the earliest tetrapoda, newly-recruited genes are increasingly paralogues of existing genes and distribute non-randomly among the chromosomes.BMC genomics · 2021Article
- Article
- The methylation and telomere landscape in two families of marsupials with different rates of chromosome evolution.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2018Article
- Marsupial chromosome DNA content and genome size assessed from flow karyotypes: invariable low autosomal GC content.Royal Society open science · 2018Article
- Adaptation and conservation insights from the koala genome.Nature genetics · 2018Article
- Chromosome Evolution in Marsupials.Genes · 2018Review
- Identification of candidate genes for devil facial tumour disease tumourigenesis.Scientific reports · 2017Article
- Marsupials as models for understanding the role of chromosome rearrangements in evolution and disease.Chromosoma · 2016Review
- Article
- The ancestral chromosomes of Dromiciops gliroides (Microbiotheridae), and its bearings on the karyotypic evolution of American marsupials.Molecular cytogenetics · 2016Article
- Physical Mapping and Refinement of the Painted Turtle Genome (Chrysemys picta) Inform Amniote Genome Evolution and Challenge Turtle-Bird Chromosomal Conservation.Genome biology and evolution · 2015Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe increasing number of assembled mammalian genomes makes it possible to compare genome organisation across mammalian lineages and reconstruct chromosomes of the ancestral marsupial and therian (marsupial and eutherian) mammals. However, the reconstruction of ancestral genomes requires genome assemblies to be anchored to chromosomes. The recently sequenced tammar wallaby (Macropus eugenii) genome was assembled into over 300,000 contigs. We previously devised an efficient strategy for mapping large evolutionarily conserved blocks in non-model mammals, and applied this to determine the arrangement of conserved blocks on all wallaby chromosomes, thereby permitting comparative maps to be constructed and resolve the long debated issue between a 2n = 14 and 2n = 22 ancestral marsupial karyotype.
resultsWe identified large blocks of genes conserved between human and opossum, and mapped genes corresponding to the ends of these blocks by fluorescence in situ hybridization (FISH). A total of 242 genes was assigned to wallaby chromosomes in the present study, bringing the total number of genes mapped to 554 and making it the most densely cytogenetically mapped marsupial genome. We used these gene assignments to construct comparative maps between wallaby and opossum, which uncovered many intrachromosomal rearrangements, particularly for genes found on wallaby chromosomes X and 3. Expanding comparisons to include chicken and human permitted the putative ancestral marsupial (2n = 14) and therian mammal (2n = 19) karyotypes to be reconstructed.
conclusionsOur physical mapping data for the tammar wallaby has uncovered the events shaping marsupial genomes and enabled us to predict the ancestral marsupial karyotype, supporting a 2n = 14 ancestor. Futhermore, our predicted therian ancestral karyotype has helped to understand the evolution of the ancestral eutherian genome.
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