ArticleJournal of thrombosis and haemostasis : JTH2014
Microfluidic assay of hemophilic blood clotting: distinct deficits in platelet and fibrin deposition at low factor levels.
Article in Journal of thrombosis and haemostasis : JTH, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- Universal pre-mixing dry-film stickers capable of retrofitting existing microfluidics.Biomicrofluidics · 2023Article
- Getting a good view:Platelets · 2020Review
- Viscoelastic Hemostatic Assays: Moving from the Laboratory to the Site of Care-A Review of Established and Emerging Technologies.Diagnostics (Basel, Switzerland) · 2020Review
- Microfluidics in Haemostasis: A Review.Molecules (Basel, Switzerland) · 2020Review
- Microfluidic hemophilia models using blood from healthy donors.Research and practice in thrombosis and haemostasis · 2020Article
- Reduced model to predict thrombin and fibrin during thrombosis on collagen/tissue factor under venous flow: Roles of γ'-fibrin and factor XIa.PLoS computational biology · 2019Article
- Contact Pathway Function During Human Whole Blood Clotting on Procoagulant Surfaces.Frontiers in medicine · 2018Review
- Recombinant factor VIIa addition to haemophilic blood perfused over collagen/tissue factor can sufficiently bypass the factor IXa/VIIIa defect to rescue fibrin generation.Haemophilia : the official journal of the World Federation of Hemophilia · 2017Article
- Clinical and laboratory phenotype variability in type 2M von Willebrand disease.Journal of thrombosis and haemostasis : JTH · 2017Article
- Review
- Flow chamber and microfluidic approaches for measuring thrombus formation in genetic bleeding disorders.Platelets · 2017Review
- Review
- Dynamics of Thrombin Generation and Flux from Clots during Whole Human Blood Flow over Collagen/Tissue Factor Surfaces.The Journal of biological chemistry · 2016Article
- Transport physics and biorheology in the setting of hemostasis and thrombosis.Journal of thrombosis and haemostasis : JTH · 2016Review
- Systems Analysis of Thrombus Formation.Circulation research · 2016Review
- Ex vivo recapitulation of trauma-induced coagulopathy and preliminary assessment of trauma patient platelet function under flow using microfluidic technology.The journal of trauma and acute care surgery · 2016Article
- Viscoelastic measurements of platelet function, not fibrinogen function, predicts sensitivity to tissue-type plasminogen activator in trauma patients.Journal of thrombosis and haemostasis : JTH · 2015Observational
- Microfluidic assessment of functional culture-derived platelets in human thrombi under flow.Experimental hematology · 2015Article
- Article
- Microfluidic technology as an emerging clinical tool to evaluate thrombosis and hemostasis.Thrombosis research · 2015Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundCoagulation factor deficiencies create a range of bleeding phenotypes. Microfluidic devices offer controlled hemodynamics and defined procoagulant triggers for measurement of clotting under flow.
objectivesWe tested a flow assay of contact pathway-triggered clotting to quantify platelet and fibrin deposition distal of dysfunctional thrombin production. Microfluidic metrics were then compared with PTT or % factor activity assays.
methodsWhole blood (WB) treated with low level corn trypsin inhibitor (4 μg mL⁻¹) from nine healthy donors and 27 patients (deficient in factor [F] VIII, 19 patients; FIX, one patient; FXI, one patient; VWF, six patients) was perfused over fibrillar collagen at wall shear rate = 100 s⁻¹.
resultsUsing healthy WB, platelets deposited within 30 s, while fibrin appeared within 6 min. Compared with healthy controls, WB from patients displayed a 50% reduction in platelet deposition only at < 1% factor activity. In contrast, striking defects in fibrin deposition occurred for patients with < 13% factor activity (or PTT > 40 s). Full occlusion of the 60-μm high channel was completely absent over the 15-min test in patients with < 1% factor activity, while an intermediate defect was present in patients with > 1% factor.
conclusionSpontaneous bleeding in patients with < 1% factor activity may be linked to deficits in both platelet and fibrin deposition, a risk known to be mitigated when factor levels are raised to > 1% activity (PTT of ~40-60 s), a level that does not necessarily rescue fibrin formation under flow.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.