ArticleStem cells and development2014
In vitro multilineage differentiation and self-renewal of single pancreatic colony-forming cells from adult C57BL/6 mice.
Article in Stem cells and development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Activation of ductal progenitor-like cells from adult human pancreas requires extracellular matrix protein signaling.iScience · 2024Article
- Pancreatic Organoids for Regenerative Medicine and Cancer Research.Frontiers in cell and developmental biology · 2022Review
- Stem/progenitor cells in normal physiology and disease of the pancreas.Molecular and cellular endocrinology · 2021Review
- Diabetes through a 3D lens: organoid models.Diabetologia · 2020Review
- Organoids from the Human Fetal and Adult Pancreas.Current diabetes reports · 2019Review
- Establishment of a high-resolution 3D modeling system for studying pancreatic epithelial cell biology in vitro.Molecular metabolism · 2019Article
- A GLIS3-CD133-WNT-signaling axis regulates the self-renewal of adult murine pancreatic progenitor-like cells in colonies and organoids.The Journal of biological chemistry · 2019Article
- Modelling the endocrine pancreas in health and disease.Nature reviews. Endocrinology · 2019Review
- α-Ketoglutarate Promotes Pancreatic Progenitor-Like Cell Proliferation.International journal of molecular sciences · 2018Article
- Culturing and transcriptome profiling of progenitor-like colonies derived from adult mouse pancreas.Stem cell research & therapy · 2017Article
- Molecular mechanisms of Sox transcription factors during the development of liver, bile duct, and pancreas.Seminars in cell & developmental biology · 2017Review
- Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels.Scientific reports · 2017Article
- In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas.Journal of visualized experiments : JoVE · 2016Article
- Growth factors and medium hyperglycemia induce Sox9+ ductal cell differentiation into β cells in mice with reversal of diabetes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Cells with surface expression of CD133highCD71low are enriched for tripotent colony-forming progenitor cells in the adult murine pancreas.Stem cell research · 2016Article
- Postnatal Pancreas of Mice Contains Tripotent Progenitors Capable of Giving Rise to Duct, Acinar, and Endocrine Cells In Vitro.Stem cells and development · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In a previous study we established colony assays suitable for studying murine adult (2-4 months) pancreatic progenitor cells plated in semisolid media containing methylcellulose and extracellular matrix proteins. Using these assays, we found robust in vitro progenitor cell activities (multilineage differentiation and self-renewal) from pancreatic cells of adult mice in the CD-1 outbred background. However, it was not clear whether progenitor cell activities can be detected from inbred mice, a preferred mouse model for various genetic studies. It was also not clear whether a single cell is sufficient to self-renew. Here, we show that fluorescent activated cell sorting pancreatic CD133(+) but not CD133(-) cells from adult C57Bl/6 inbred mice are enriched for progenitor cells that self-renew and give rise to multilineage colonies in vitro. The number of cells in a colony is in proportion to its diameter. Around 60% of single handpicked 3-week-old colonies express trilineage markers, indicating most progenitors are tripotent for ductal, acinar, and endocrine lineage differentiation. Approximately 80% of primary (freshly sorted) colony-forming progenitor cells are capable of giving rise to secondary progenitors in vitro, indicating that a majority of the primary progenitors self-renew. A single cell is sufficient for self-renewal and a Wnt agonist, R-Spondin1, enhances the number of secondary progenitors from the primary progenitors. Together, our pancreatic colony assays allow quantitative analyses of progenitors at a single-cell level from inbred mice. These assays will be useful for elucidating in vitro mechanisms of pancreatic progenitor cell biology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.