ArticlePloS one2013
Transcriptomic analysis reveals novel mechanistic insight into murine biological responses to multi-walled carbon nanotubes in lungs and cultured lung epithelial cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Applicability of in vitro mouse lung epithelial cell responses for potency grouping and hazard identification of metal oxide nanoparticles: impact of form, size, surface area, and solubility on toxicity.Archives of toxicology · 2026Article
- Detection of Microbial Contamination in Nanomaterials Using LAL, rFC and Cell-Based Assays: Implications for Nanotoxicological Hazard Assessment.Nanomaterials (Basel, Switzerland) · 2025Article
- Fiber length and shape-dependent differences in hepatic nanomaterial localization in mice following pulmonary exposure.Particle and fibre toxicology · 2025Article
- Towards a harmonized testing strategy for nanofibers by integrating toxicological screening and proteomic profiling.Scientific reports · 2025Article
- Acute Toxicity of Metal Oxide Nanoparticles-Role of Intracellular Localization In Vitro in Lung Epithelial Cells.International journal of molecular sciences · 2025Article
- Imogolite nanotube modifications impact pulmonary toxicity in mice: implications for safe and sustainable by design (SSbD).Journal of nanobiotechnology · 2025Article
- Toxicity of Metal Oxide Nanoparticles: Looking through the Lens of Toxicogenomics.International journal of molecular sciences · 2023Article
- Time-Course of Transcriptomic Change in the Lungs of F344 Rats Repeatedly Exposed to a Multiwalled Carbon Nanotube in a 2-Year Test.Nanomaterials (Basel, Switzerland) · 2023Article
- Single-Walled vs. Multi-Walled Carbon Nanotubes: Influence of Physico-Chemical Properties on Toxicogenomics Responses in Mouse Lungs.Nanomaterials (Basel, Switzerland) · 2023Article
- Role of RNA modifications in carcinogenesis and carcinogen damage response.Molecular carcinogenesis · 2023Article
- Toxicogenomics Data for Chemical Safety Assessment and Development of New Approach Methodologies: An Adverse Outcome Pathway-Based Approach.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Do Carbon Nanotubes and Asbestos Fibers Exhibit Common Toxicity Mechanisms?Nanomaterials (Basel, Switzerland) · 2022Review
- Innate but Not Adaptive Immunity Regulates Lung Recovery from Chronic Exposure to Graphene Oxide Nanosheets.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Nanomaterial- and shape-dependency of TLR2 and TLR4 mediated signaling following pulmonary exposure to carbonaceous nanomaterials in mice.Particle and fibre toxicology · 2021Article
- In vitro-in vivo correlations of pulmonary inflammogenicity and genotoxicity of MWCNT.Particle and fibre toxicology · 2021Article
- Evaluation ofIranian journal of biotechnology · 2021Article
- Manually curated transcriptomics data collection for toxicogenomic assessment of engineered nanomaterials.Scientific data · 2021Article
- Transcriptomics in Toxicogenomics, Part I: Experimental Design, Technologies, Publicly Available Data, and Regulatory Aspects.Nanomaterials (Basel, Switzerland) · 2020Review
- A novel human 3D lung microtissue model for nanoparticle-induced cell-matrix alterations.Particle and fibre toxicology · 2019Article
- Advances in Nano Neuroscience: From Nanomaterials to Nanotools.Frontiers in neuroscience · 2018Review
Corrections and comments
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Authors and funding
16 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is great interest in substituting animal work with in vitro experimentation in human health risk assessment; however, there are only few comparisons of in vitro and in vivo biological responses to engineered nanomaterials. We used high-content genomics tools to compare in vivo pulmonary responses of multiwalled carbon nanotubes (MWCNT) to those in vitro in cultured lung epithelial cells (FE1) at the global transcriptomic level. Primary size, surface area and other properties of MWCNT- XNRI -7 (Mitsui7) were characterized using DLS, SEM and TEM. Mice were exposed via a single intratracheal instillation to 18, 54, or 162 μg of Mitsui7/mouse. FE1 cells were incubated with 12.5, 25 and 100 μg/ml of Mitsui7. Tissue and cell samples were collected at 24 hours post-exposure. DNA microarrays were employed to establish mechanistic differences and similarities between the two models. Microarray results were confirmed using gene-specific RT-qPCR. Bronchoalveolar lavage (BAL) fluid was assessed for indications of inflammation in vivo. A strong dose-dependent activation of acute phase and inflammation response was observed in mouse lungs reflective mainly of an inflammatory response as observed in BAL. In vitro, a wide variety of core cellular functions were affected including transcription, cell cycle, and cellular growth and proliferation. Oxidative stress, fibrosis and inflammation processes were altered in both models. Although there were similarities observed between the two models at the pathway-level, the specific genes altered under these pathways were different, suggesting that the underlying mechanisms of responses are different in cells in culture and the lung tissue. Our results suggest that careful consideration should be given in selecting relevant endpoints when substituting animal with in vitro testing.
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