ArticlePloS one2013
Generation of doubled haploid transgenic wheat lines by microspore transformation.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 60 citations in OpenAlex.
- Bread Wheat Anther Culture.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Anther Culture Protocols for Barley and Wheat.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- Callus Induction from Diverse Explants and Genotypes Enables Robust Transformation of Perennial Ryegrass (Plants (Basel, Switzerland) · 2022Article
- Effects of genotype and culture conditions on microspore embryogenesis in radish (Molecular breeding : new strategies in plant improvement · 2022Article
- Glutathione and copper ions as critical factors of green plant regeneration efficiency of triticaleFrontiers in plant science · 2022Article
- Plant biomacromolecule delivery methods in the 21st century.Frontiers in genome editing · 2022Review
- Androgenesis-Based Doubled Haploidy: Past, Present, and Future Perspectives.Frontiers in plant science · 2021Review
- Homozygous Transgenic Barley (Plants (Basel, Switzerland) · 2020Article
- In vitro androgenesis: spontaneous vs. artificial genome doubling and characterization of regenerants.Plant cell reports · 2020Review
- The Effect of Caffeine and Trifluralin on Chromosome Doubling in Wheat Anther Culture.Plants (Basel, Switzerland) · 2020Article
- Genetic Modification for Wheat Improvement: From Transgenesis to Genome Editing.BioMed research international · 2019Review
- Regeneration of highland papaya (Applications in plant sciences · 2018Article
- Targeted mutagenesis in wheat microspores using CRISPR/Cas9.Scientific reports · 2018Article
- Application of CRISPR/Cas9 Genome Editing Technology for the Improvement of Crops Cultivated in Tropical Climates: Recent Progress, Prospects, and Challenges.Frontiers in plant science · 2018Review
- Molecular characterization and expression analysis of SERK1 and SERK2 in Brassica napus L.: implication for microspore embryogenesis and plant regeneration.Plant cell reports · 2016Article
- Intracellular delivery of fluorescent protein into viable wheat microspores using cationic peptides.Frontiers in plant science · 2015Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Microspores can be induced to develop homozygous doubled haploid plants in a single generation. In the present experiments androgenic microspores of wheat have been genetically transformed and developed into mature homozygous transgenic plants. Two different transformation techniques were investigated, one employing electroporation and the other co-cultivation with Agrobacterium tumefaciens. Different tissue culture and transfection conditions were tested on nine different wheat cultivars using four different constructs. A total of 19 fertile transformants in five genotypes from four market classes of common wheat were recovered by the two procedures. PCR followed by DNA sequencing of the products, Southern blot analyses and bio/histo-chemical and histological assays of the recombinant enzymes confirmed the presence of the transgenes in the T0 transformants and their stable inheritance in homozygous T1∶2 doubled haploid progenies. Several decisive factors determining the transformation and regeneration efficiency with the two procedures were determined: (i) pretreatment of immature spikes with CuSO4 solution (500 mg/L) at 4°C for 10 days; (ii) electroporation of plasmid DNA in enlarged microspores by a single pulse of ∼375 V; (iii) induction of microspores after transfection at 28°C in NPB-99 medium and regeneration at 26°C in MMS5 medium; (iv) co-cultivation with Agrobacterium AGL-1 cells for transfer of plasmid T-DNA into microspores at day 0 for <24 hours; and (v) elimination of AGL-1 cells after co-cultivation with timentin (200-400 mg/L).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.