ArticlePloS one2013
Unraveling the role of surface mucus-binding protein and pili in muco-adhesion of Lactococcus lactis.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- Astrovirus infection alters gut microbial communities in a widespread neotropical bat across human-modified landscapes.BMC microbiology · 2026Article
- Screening for probiotic properties and potential immunogenic effects of lactobacilli strains isolated from various food products.Frontiers in microbiology · 2024Article
- Intestinal mucus and their glycans: A habitat for thriving microbiota.Cell host & microbe · 2023Review
- Health-Promoting Nature ofFrontiers in microbiology · 2022Article
- Elucidating bacterial adhesion to mucosal surface by an original AFM approach.BMC microbiology · 2021Article
- Pili and other surface proteins influence the structure and the nanomechanical properties of Lactococcus lactis biofilms.Scientific reports · 2021Article
- Review
- Quality control of direct cell-mineral adhesion measurements in air and liquid using inverse AFM imaging.RSC advances · 2021Article
- Quantifying and Engineering Mucus Adhesion of Probiotics.ACS synthetic biology · 2020Article
- Atomic Force Microscopy (AFM) As a Surface Mapping Tool in Microorganisms Resistant Toward Antimicrobials: A Mini-Review.Frontiers in pharmacology · 2020Review
- Metabolic engineering of Lactococcus lactis for high level accumulation of glutathione and S-adenosyl-L-methionine.World journal of microbiology & biotechnology · 2019Article
- TheFrontiers in microbiology · 2019Article
- The Role of Glycans in Bacterial Adhesion to Mucosal Surfaces: How Can Single-Molecule Techniques Advance Our Understanding?Microorganisms · 2018Review
- Article
- Characterization of Mucus-Related Properties ofFrontiers in physiology · 2018Article
- Contribution of plasmid-encoded peptidase S8 (PrtP) to adhesion and transit in the gut of Lactococcus lactis IBB477 strain.Applied microbiology and biotechnology · 2017Article
- From Genome to Phenotype: An Integrative Approach to Evaluate the Biodiversity of Lactococcus lactis.Microorganisms · 2017Review
- Surface Proteins ofFrontiers in microbiology · 2017Review
- Interactions of Intestinal Bacteria with Components of the Intestinal Mucus.Frontiers in cellular and infection microbiology · 2017Review
- Use of Atomic Force Microscopy to Study the Multi-Modular Interaction of Bacterial Adhesins to Mucins.International journal of molecular sciences · 2016Article
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adhesion of bacteria to mucus may favor their persistence within the gut and their beneficial effects to the host. Interactions between pig gastric mucin (PGM) and a natural isolate of Lactococcus lactis (TIL448) were measured at the single-cell scale and under static conditions, using atomic force microscopy (AFM). In parallel, these interactions were monitored at the bacterial population level and under shear flow. AFM experiments with a L. lactis cell-probe and a PGM-coated surface revealed a high proportion of specific adhesive events (60%) and a low level of non-adhesive ones (2%). The strain muco-adhesive properties were confirmed by the weak detachment of bacteria from the PGM-coated surface under shear flow. In AFM, rupture events were detected at short (100-200 nm) and long distances (up to 600-800 nm). AFM measurements on pili and mucus-binding protein defective mutants demonstrated the comparable role played by these two surface proteinaceous components in adhesion to PGM under static conditions. Under shear flow, a more important contribution of the mucus-binding protein than the pili one was observed. Both methods differ by the way of probing the adhesion force, i.e. negative force contact vs. sedimentation and normal-to-substratum retraction vs. tangential detachment conditions, using AFM and flow chamber, respectively. AFM blocking assays with free PGM or O-glycan fractions purified from PGM demonstrated that neutral oligosaccharides played a major role in adhesion of L. lactis TIL448 to PGM. This study dissects L. lactis muco-adhesive phenotype, in relation with the nature of the bacterial surface determinants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.