ArticlePloS one2013
Improved cell line IPEC-J2, characterized as a model for porcine jejunal epithelium.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 106 citations in OpenAlex.
- Trial
- The anti-coagulating agent heparin and native serum alter the barrier and tight junction properties of a primary cultured model fish gill epithelium.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2026Article
- Establishment of a Porcine Small Intestinal Organoid Model for Porcine Rotavirus Infection.Animals : an open access journal from MDPI · 2026Article
- Culture Optimization of the IPEC-J2 Piglet Jejunum Cell Line for Applications in Infant Nutrition Research.Molecular nutrition & food research · 2026Article
- Investigating alterations associated with heat stress and the recovery of the intestinal barrier using IPEC-J2 as an intestinal epithelial porcine cell model.Scientific reports · 2026Article
- Effects of illite or bentonite on cytotoxicity, antibacterial and adsorption capacity in porcine intestinal epithelial cells.Journal of animal science and technology · 2026Article
- Functional characteristics of a pig 2D intestinal organoid model as anJournal of animal science and technology · 2026Article
- IPEC-J2 as a cellular model for studying intestinal mucus.Scientific reports · 2025Article
- Relevance of porcine intestinal organoids as a surrogate for animal experimentation: application to the investigation of host-virus interactions during porcine coronavirus infection.Veterinary research · 2025Article
- Milk-Derived Extracellular Vesicles and microRNAs: Potential Modulators of Intestinal Homeostasis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Review
- Serotype 2 Streptococcus suis growth inhibition mediated by intraspecies contact-dependent mechanism.Scientific reports · 2025Article
- Butyrate Derivatives Exhibited Anti-Inflammatory Effects and Enhanced Intestinal Barrier Integrity in Porcine Cell Culture Models.Animals : an open access journal from MDPI · 2025Article
- Article
- Matrix-free human 2D organoids recapitulate duodenal barrier and transport properties.BMC biology · 2025Article
- Comprehensive evaluation of a pea co-product for piglet nutrition: fibre content, protein digestion, and intestinal barrier function.Journal of animal science · 2025Article
- Tumour necrosis factor-α induces macromolecule translocation in HIV-derived duodenal organoids.Frontiers in immunology · 2025Article
- Growth behavior and mRNA expression profiling during growth of IPEC-J2 cells.BMC research notes · 2024Article
- Cortactin-dependent control of Par1b-regulated epithelial cell polarity inCell insight · 2024Article
- Generation of Porcine and Rainbow Trout 3D Intestinal Models and Their Use to Investigate Astaxanthin Effects In Vitro.International journal of molecular sciences · 2024Article
- Microbial Community and Metabolome Analysis of the Porcine Intestinal Damage Model Induced by the IPEC-J2 Cell Culture-Adapted Porcine Deltacoronavirus (PDCoV) Infection.Microorganisms · 2024Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell lines matching the source epithelium are indispensable for investigating porcine intestinal transport and barrier properties on a subcellular or molecular level and furthermore help to reduce animal usage. The porcine jejunal cell line IPEC-J2 is established as an in vitro model for porcine infection studies but exhibits atypically high transepithelial resistances (TER) and only low active transport rates so that the effect of nutritional factors cannot be reliably investigated. This study aimed to properly remodel IPEC-J2 and then to re-characterize these cells regarding epithelial architecture, expression of barrier-relevant tight junction (TJ) proteins, adequate TER and transport function, and reaction to secretagogues. For this, IPEC-J2 monolayers were cultured on permeable supports, either under conventional (fetal bovine serum, FBS) or species-specific (porcine serum, PS) conditions. Porcine jejunal mucosa was analyzed for comparison. Main results were that under PS conditions (IPEC-J2/PS), compared to conventional FBS culture (IPEC-J2/FBS), the cell height increased 6-fold while the cell diameter was reduced by 50%. The apical cell membrane of IPEC-J2/PS exhibited typical microvilli. Most importantly, PS caused a one order of magnitude reduction of TER and of trans- and paracellular resistance, and a 2-fold increase in secretory response to forskolin when compared to FBS condition. TJ ultrastructure and appearance of TJ proteins changed dramatically in IPEC-J2/PS. Most parameters measured under PS conditions were much closer to those of typical pig jejunocytes than ever reported since the cell line's initial establishment in 1989. In conclusion, IPEC-J2, if cultured under defined species-specific conditions, forms a suitable model for investigating porcine paracellular intestinal barrier function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.