ArticlePloS one2013
An intergenic region shared by At4g35985 and At4g35987 in Arabidopsis thaliana is a tissue specific and stress inducible bidirectional promoter analyzed in transgenic arabidopsis and tobacco plants.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 60 citations in OpenAlex.
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- Characterization of theInternational journal of molecular sciences · 2023Article
- Uncovering the role of wheat magnesium transporter family genes in abiotic responses.Frontiers in plant science · 2023Article
- Isolation and characterization of drought and ABA responsive promoter of a transcription factor encoding gene from rice.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Exogenous Melatonin Improves Seed Germination of Wheat (International journal of molecular sciences · 2022Article
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- Genome-Wide Identification and Characterization of the Brassinazole-resistant (International journal of molecular sciences · 2021Article
- Genome-Wide Identification and Characterization of PIN-FORMED (PIN) Gene Family Reveals Role in Developmental and Various Stress Conditions inInternational journal of molecular sciences · 2021Article
- Dissecting the Role of Promoters of Pathogen-sensitive Genes in Plant Defense.Current genomics · 2020Review
- Functional characterization of an inducible bidirectional promoter from Fusarium oxysporum f. sp. cubense.Scientific reports · 2020Article
- Molecular Characterization, Expression Pattern and Function Analysis of Glycine-Rich Protein Genes Under Stresses in Chinese Cabbage (Frontiers in genetics · 2020Article
- An update and perspectives on the use of promoters in plant genetic engineering.Journal of biosciences · 2020Review
- Plant Defenses Against Pests Driven by a Bidirectional Promoter.Frontiers in plant science · 2019Article
- Structural and Functional Analysis of a Bidirectional Promoter fromInternational journal of molecular sciences · 2018Article
- Analysis of Promoters of Arabidopsis thaliana Divergent Gene Pair SERAT3;2 and IDH-III Shows SERAT3;2 Promoter is Nested Within the IDH-III Promoter.Molecular biotechnology · 2017Article
- A phosphate starvation-driven bidirectional promoter as a potential tool for crop improvement and in vitro plant biotechnology.Plant biotechnology journal · 2017Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
On chromosome 4 in the Arabidopsis genome, two neighboring genes (calmodulin methyl transferase At4g35987 and senescence associated gene At4g35985) are located in a head-to-head divergent orientation sharing a putative bidirectional promoter. This 1258 bp intergenic region contains a number of environmental stress responsive and tissue specific cis-regulatory elements. Transcript analysis of At4g35985 and At4g35987 genes by quantitative real time PCR showed tissue specific and stress inducible expression profiles. We tested the bidirectional promoter-function of the intergenic region shared by the divergent genes At4g35985 and At4g35987 using two reporter genes (GFP and GUS) in both orientations in transient tobacco protoplast and Agro-infiltration assays, as well as in stably transformed transgenic Arabidopsis and tobacco plants. In transient assays with GFP and GUS reporter genes the At4g35985 promoter (P85) showed stronger expression (about 3.5 fold) compared to the At4g35987 promoter (P87). The tissue specific as well as stress responsive functional nature of the bidirectional promoter was evaluated in independent transgenic Arabidopsis and tobacco lines. Expression of P85 activity was detected in the midrib of leaves, leaf trichomes, apical meristemic regions, throughout the root, lateral roots and flowers. The expression of P87 was observed in leaf-tip, hydathodes, apical meristem, root tips, emerging lateral root tips, root stele region and in floral tissues. The bidirectional promoter in both orientations shows differential up-regulation (2.5 to 3 fold) under salt stress. Use of such regulatory elements of bidirectional promoters showing spatial and stress inducible promoter-functions in heterologous system might be an important tool for plant biotechnology and gene stacking applications.
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