Trial reportPloS one2013
Optical imaging of human cone photoreceptors directly following the capture of light.
Trial report in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed.
- Evaluating Human Rod Photoreceptor Function Using Pixelwise Intensity-Based Optoretinography.Translational vision science & technology · 2026Article
- ƒ(Cell): Software for Reproducible Analysis of Optoretinograms.Translational vision science & technology · 2026Article
- Recent Developments in Photoreceptor Optoretinography and Progress Toward Clinical Use.Translational vision science & technology · 2025Review
- Visual evoked potentials in patients with congenital color vision deficiency.International ophthalmology · 2024Article
- Validation of an automated method for studying retinal capillary blood flow.Biomedical optics express · 2024Article
- Flow Heterogeneity and Factors Contributing to the Variability in Retinal Capillary Blood Flow.Investigative ophthalmology & visual science · 2023Article
- Evolution of adaptive optics retinal imaging [Invited].Biomedical optics express · 2023Review
- Mapping the human parafoveal vascular network to understand flow variability in capillaries.PloS one · 2023Article
- Characterizing cone spectral classification by optoretinography.Biomedical optics express · 2022Article
- Assessment of photoreceptor function with ultrafast retinal densitometry.Biomedical optics express · 2022Article
- Extraction of phase-based optoretinograms (ORG) from serial B-scans acquired over tens of seconds by mouse retinal raster scanning OCT system.Biomedical optics express · 2021Article
- Structural evaluation in inherited retinal diseases.The British journal of ophthalmology · 2021Review
- Reflective mirror-based line-scan adaptive optics OCT for imaging retinal structure and function.Biomedical optics express · 2021Article
- Functional intrinsic optical signal imaging for objective optoretinography of human photoreceptors.Experimental biology and medicine (Maywood, N.J.) · 2021Article
- Revealing How Color Vision Phenotype and Genotype Manifest in Individual Cone Cells.Investigative ophthalmology & visual science · 2021Article
- Kilohertz retinal FF-SS-OCT and flood imaging with hardware-based adaptive optics.Biomedical optics express · 2020Article
- High-speed adaptive optics line-scan OCT for cellular-resolution optoretinography.Biomedical optics express · 2020Article
- Fast intrinsic optical signal correlates with activation phase of phototransduction in retinal photoreceptors.Experimental biology and medicine (Maywood, N.J.) · 2020Review
- Cone photoreceptor classification in the living human eye from photostimulation-induced phase dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Adaptive optics imaging of the human retina.Progress in retinal and eye research · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Capture of light in the photoreceptor outer segment initiates a cascade of chemical events that inhibit neurotransmitter release, ultimately resulting in vision. The massed response of the photoreceptor population can be measured non-invasively by electrical recordings, but responses from individual cells cannot be measured without dissecting the retina. Here we used optical imaging to observe individual human cones in the living eye as they underwent bleaching of photopigment and associated phototransduction. The retina was simultaneously stimulated and observed with high intensity visible light at 1 kHz, using adaptive optics. There was marked variability between individual cones in both photosensitivity and pigment optical density, challenging the conventional assumption that photoreceptors act as identical subunits (coefficient of variation in rate of photoisomerization = 23%). There was also a pronounced inverse correlation between these two parameters (p<10(-7)); the temporal evolution of image statistics revealed this to be a dynamic relationship, with cone waveguiding efficiency beginning a dramatic increase within 3 ms of light onset. Beginning as early as 2 ms after light onset and including half of cells by ∼7 ms, cone intensity showed reversals characteristic of interference phenomena, with greater delays in reversal corresponding to cones with more photopigment (p<10(-3)). The timing of these changes is argued to best correspond with either the cessation of dark current, or to related events such as changes in intracellular cGMP. Cone intensity also showed fluctuations of high frequency (332±25 Hz) and low amplitude (3.0±0.85%). Other groups have shown similar fluctuations that were directly evoked by light; if this corresponds to the same phenomenon, we propose that the amplitude of fluctuation may be increased by the use of a bright flash followed by a brief pause, to allow recovery of cone circulating current.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.