ArticlePloS one2013
Overexpression of cytokinin dehydrogenase genes in barley (Hordeum vulgare cv. Golden Promise) fundamentally affects morphology and fertility.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 62 citations in OpenAlex.
- Cultivar-dependent regulation of cytokinin biosynthesis in wheat: developmental expression of TaIPT genes and hormonal crosstalk during reproductive development.BMC plant biology · 2026Article
- Circadian clock regulation in soybean senescence: a transcriptome analysis of early and late senescence types.BMC genomics · 2025Article
- Cytokinin Translocation to, and Biosynthesis and Metabolism within, Cereal and Legume Seeds: Looking Back to Inform the Future.Metabolites · 2023Review
- SWEET11b transports both sugar and cytokinin in developing barley grains.The Plant cell · 2023Article
- ImprovingGenes · 2023Article
- Cytokinin Oxygenase/Dehydrogenase Inhibitors: An Emerging Tool in Stress Biotechnology Employed for Crop Improvement.Frontiers in genetics · 2022Review
- Cytokinins: A Genetic Target for Increasing Yield Potential in the CRISPR Era.Frontiers in genetics · 2022Review
- Genome-wide identification and analysis of the cytokinin oxidase/dehydrogenase (Frontiers in genetics · 2022Article
- Cytokinin glucosyl transferases, key regulators of cytokinin homeostasis, have potential value for wheat improvement.Plant biotechnology journal · 2021Review
- The Hulks and the Deadpools of the Cytokinin Universe: A Dual Strategy for Cytokinin Production, Translocation, and Signal Transduction.Biomolecules · 2021Review
- CytokininBiomolecules · 2020Article
- Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato.Plant methods · 2020Article
- TaCKX gene family, at large, is associated with thousand-grain weight and plant height in common wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Different sets of TaCKX genes affect yield-related traits in wheat plants grown in a controlled environment and in field conditions.BMC plant biology · 2020Article
- The HK5 and HK6 cytokinin receptors mediate diverse developmental pathways in rice.Development (Cambridge, England) · 2020Article
- Comparative Transcriptomic Analysis of the Development of Sepal Morphology in Tomato (International journal of molecular sciences · 2020Article
- Expression and Role of Response Regulating, Biosynthetic and Degrading Genes for Cytokinin Signaling during Clubroot Disease Development.International journal of molecular sciences · 2020Article
- Cytokinin dehydrogenase: a genetic target for yield improvement in wheat.Plant biotechnology journal · 2020Review
- Barley Root Proteome and Metabolome in Response to Cytokinin and Abiotic Stimuli.Frontiers in plant science · 2020Article
- Knockout of theCells · 2019Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Barley is one of the most important cereal crops grown worldwide. It has numerous applications, but its utility could potentially be extended by genetically manipulating its hormonal balances. To explore some of this potential we identified gene families of cytokinin dehydrogenases (CKX) and isopentenyl transferases, enzymes that respectively irreversibly degrade and synthesize cytokinin (CK) plant hormones, in the raw sequenced barley genome. We then examined their spatial and temporal expression patterns by immunostaining and qPCR. Two CKX-specific antibodies, anti-HvCKX1 and anti-HvCKX9, predominantly detect proteins in the aleurone layer of maturing grains and leaf vasculature, respectively. In addition, two selected CKX genes were used for stable, Agrobacterium tumefaciens-mediated transformation of the barley cultivar Golden Promise. The results show that constitutive overexpression of CKX causes morphological changes in barley plants and prevents their transition to flowering. In all independent transgenic lines roots proliferated more rapidly and root-to-shoot ratios were higher than in wild-type plants. Only one transgenic line, overexpressing CKX under the control of a promoter from a phosphate transporter gene, which is expressed more strongly in root tissue than in aerial parts, yielded progeny. Analysis of several T1-generation plants indicates that plants tend to compensate for effects of the transgene and restore CK homeostasis later during development. Depleted CK levels during early phases of development are restored by down-regulation of endogenous CKX genes and reinforced de novo biosynthesis of CKs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.