ArticleNucleic acids research2014
Trans-splicing and RNA editing of LSU rRNA in Diplonema mitochondria.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- The mitochondrial proteome of diplonemids: from conventional pathways to eccentric RNA editing and transcript processing.BMC genomics · 2025Article
- Mitochondrial genomes revisited: why do different lineages retain different genes?BMC biology · 2024Review
- Miniature RNAs are embedded in an exceptionally protein-rich mitoribosome via an elaborate assembly pathway.Nucleic acids research · 2023Article
- Typical structure of rRNA coding genes in diplonemids points to two independent origins of the bizarre rDNA structures of euglenozoans.BMC ecology and evolution · 2022Article
- An Unexpectedly Complex Mitoribosome in Andalucia godoyi, a Protist with the Most Bacteria-like Mitochondrial Genome.Molecular biology and evolution · 2021Article
- Gene fragmentation and RNA editing without borders: eccentric mitochondrial genomes of diplonemids.Nucleic acids research · 2020Article
- The architecture of the Plasmodiophora brassicae nuclear and mitochondrial genomes.Scientific reports · 2019Article
- Respiratory chain Complex I of unparalleled divergence in diplonemids.The Journal of biological chemistry · 2018Article
- Pervasive, Genome-Wide Transcription in the Organelle Genomes of Diverse Plastid-Bearing Protists.G3 (Bethesda, Md.) · 2017Article
- Keeping it complicated: Mitochondrial genome plasticity across diplonemids.Scientific reports · 2017Article
- Post-transcriptional mending of gene sequences: Looking under the hood of mitochondrial gene expression in diplonemids.RNA biology · 2016Review
- Animal Mitochondrial DNA as We Do Not Know It: mt-Genome Organization and Evolution in Nonbilaterian Lineages.Genome biology and evolution · 2016Article
- Hyper-eccentric structural genes in the mitochondrial genome of the algal parasite Hemistasia phaeocysticola.Genome biology and evolution · 2016Article
- Novel modes of RNA editing in mitochondria.Nucleic acids research · 2016Article
- The Extent of mRNA Editing Is Limited in Chicken Liver and Adipose, but Impacted by Tissular Context, Genotype, Age, and Feeding as Exemplified with a Conserved Edited Site in COG3.G3 (Bethesda, Md.) · 2015Article
- Article
- Unexpectedly Streamlined Mitochondrial Genome of the Euglenozoan Euglena gracilis.Genome biology and evolution · 2015Article
- Three-dimensional structure model and predicted ATP interaction rewiring of a deviant RNA ligase 2.BMC structural biology · 2015Article
- Trypanosome RNA editing: the complexity of getting U in and taking U out.Wiley interdisciplinary reviews. RNAReview
- Stunning Intricacies of RNA Editing Complexes RECC, RESC, and REH2C: Functional Organization, Developmental Regulation, and Evolutionary History in Kinetoplastid Protists.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mitochondrial ribosomal RNAs (rRNAs) often display reduced size and deviant secondary structure, and sometimes are fragmented, as are their corresponding genes. Here we report a mitochondrial large subunit rRNA (mt-LSU rRNA) with unprecedented features. In the protist Diplonema, the rnl gene is split into two pieces (modules 1 and 2, 534- and 352-nt long) that are encoded by distinct mitochondrial chromosomes, yet the rRNA is continuous. To reconstruct the post-transcriptional maturation pathway of this rRNA, we have catalogued transcript intermediates by deep RNA sequencing and RT-PCR. Gene modules are transcribed separately. Subsequently, transcripts are end-processed, the module-1 transcript is polyuridylated and the module-2 transcript is polyadenylated. The two modules are joined via trans-splicing that retains at the junction ∼ 26 uridines, resulting in an extent of insertion RNA editing not observed before in any system. The A-tail of trans-spliced molecules is shorter than that of mono-module 2, and completely absent from mitoribosome-associated mt-LSU rRNA. We also characterize putative antisense transcripts. Antisense-mono-modules corroborate bi-directional transcription of chromosomes. Antisense-mt-LSU rRNA, if functional, has the potential of guiding concomitantly trans-splicing and editing of this rRNA. Together, these findings open a window on the investigation of complex regulatory networks that orchestrate multiple and biochemically diverse post-transcriptional events.
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