ReviewProtoplasma2014
Out with the old and in with the new: rapid specimen preparation procedures for electron microscopy of sectioned biological material.
Review in Protoplasma, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 108 citations in OpenAlex.
- Optimisation of freeze substitution protocols for the examination of malaria parasite structure by volumetric electron microscopy.Journal of microscopy · 2026Article
- Tom70-mediated mitochondria-nuclear envelope contacts regulate nuclear pore complex inheritance during gametogenesis.bioRxiv : the preprint server for biology · 2025Article
- GRANA: An AI-based tool for accelerating chloroplast grana nanomorphology analysis using hybrid intelligence.Plant physiology · 2025Article
- Article
- Advancing yeast cell analysis: A cryomethod for serial block-face scanning electron microscopy imaging in mitochondrial morphology studies.Biology of the cell · 2025Article
- A comprehensive overview of focused ion beam-scanning electron microscopy (FIB-SEM) applications for the evaluation of outer retina.Frontiers in cell and developmental biology · 2025Review
- Enteric glia regulate Paneth cell secretion and intestinal microbial ecology.bioRxiv : the preprint server for biology · 2024Article
- Four-dimensional quantitative analysis of cell plate development in Arabidopsis using lattice light sheet microscopy identifies robust transition points between growth phases.Journal of experimental botany · 2024Article
- Cryoimmobilized anther analysis reveals new ultrastructural insights intoFrontiers in plant science · 2024Article
- Ultrastructure of the nebenkern during spermatogenesis in the praying mantid Hierodula membranacea.PloS one · 2023Article
- Irregular particle morphology and membrane rupture facilitate ion gradients in the lumen of phagosomes.Biophysical reports · 2022Article
- Volume electron microscopy.Nature reviews. Methods primers · 2022Article
- The ESCRT machinery directs quality control over inner nuclear membrane architecture.Cell reports · 2022Article
- A versatile enhanced freeze-substitution protocol for volume electron microscopy.Frontiers in cell and developmental biology · 2022Article
- Focused Ion Beam-Scanning Electron Microscopy for Investigating Plasmodesmal Densities.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- Cytonemes with complex geometries and composition extend into invaginations of target cells.The Journal of cell biology · 2021Article
- TheJournal of fungi (Basel, Switzerland) · 2021Article
- Current Microscopy Strategies to Image Fungal Vesicles: From the Intracellular Trafficking and Secretion to the Inner Structure of Isolated Vesicles.Current topics in microbiology and immunology · 2021Article
- How to Measure Grana - Ultrastructural Features of Thylakoid Membranes of Plant Chloroplasts.Frontiers in plant science · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This article presents the best current practices for preparation of biological samples for examination as thin sections in an electron microscope. The historical development of fixation, dehydration, and embedding procedures for biological materials are reviewed for both conventional and low temperature methods. Conventional procedures for processing cells and tissues are usually done over days and often produce distortions, extractions, and other artifacts that are not acceptable for today's structural biology standards. High-pressure freezing and freeze substitution can minimize some of these artifacts. New methods that reduce the times for freeze substitution and resin embedding to a few hours are discussed as well as a new rapid room temperature method for preparing cells for on-section immunolabeling without the use of aldehyde fixatives.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.