ArticleOpen biology2013
RNA-binding protein GLD-1/quaking genetically interacts with the mir-35 and the let-7 miRNA pathways in Caenorhabditis elegans.
Article in Open biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Article
- ADR-2 regulates fertility and oocyte fate inbioRxiv : the preprint server for biology · 2024Article
- Recent advances in understanding microRNA function and regulation in C. elegans.Seminars in cell & developmental biology · 2024Review
- Genetic and Chemical Controls of Sperm Fate and Spermatocyte Dedifferentiation via PUF-8 and MPK-1 inCells · 2023Article
- A Proposed Role for Interactions between Argonautes, miRISC, and RNA Binding Proteins in the Regulation of Local Translation in Neurons and Glia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Review
- Germ granule dysfunction is a hallmark and mirror of Piwi mutant sterility.Nature communications · 2021Article
- KH domain containing RNA-binding proteins coordinate with microRNAs to regulate Caenorhabditis elegans development.G3 (Bethesda, Md.) · 2021Article
- The RNA-binding protein QKI-7 recruits the poly(A) polymerase GLD-2 for 3' adenylation and selective stabilization of microRNA-122.The Journal of biological chemistry · 2020Article
- HRPK-1, a conserved KH-domain protein, modulates microRNA activity during Caenorhabditis elegans development.PLoS genetics · 2019Article
- Identification of proteins and miRNAs that specifically bind an mRNA in vivo.Nature communications · 2019Article
- RNA interference may result in unexpected phenotypes in Caenorhabditis elegans.Nucleic acids research · 2019Article
- VIG-1 is required for maintenance of genome stability inAnimal cells and systems · 2018Article
- Tag team: Roles of miRNAs and Proteolytic Regulators in Ensuring Robust Gene Expression Dynamics.Trends in genetics : TIG · 2018Review
- RNA Binding Protein Vigilin Collaborates with miRNAs To Regulate Gene Expression forG3 (Bethesda, Md.) · 2017Article
- The Helicase Aquarius/EMB-4 Is Required to Overcome Intronic Barriers to Allow Nuclear RNAi Pathways to Heritably Silence Transcription.Developmental cell · 2017Article
- TEG-1 CD2BP2 controls miRNA levels by regulating miRISC stability in C. elegans and human cells.Nucleic acids research · 2017Article
- Small RNA in situ hybridization in Caenorhabditis elegans, combined with RNA-seq, identifies germline-enriched microRNAs.Developmental biology · 2016Article
- Poly(A)-binding proteins are required for microRNA-mediated silencing and to promote target deadenylation in C. elegans.Nucleic acids research · 2016Article
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
Messenger RNA translation is regulated by RNA-binding proteins and small non-coding RNAs called microRNAs. Even though we know the majority of RNA-binding proteins and microRNAs that regulate messenger RNA expression, evidence of interactions between the two remain elusive. The role of the RNA-binding protein GLD-1 as a translational repressor is well studied during Caenorhabditis elegans germline development and maintenance. Possible functions of GLD-1 during somatic development and the mechanism of how GLD-1 acts as a translational repressor are not known. Its human homologue, quaking (QKI), is essential for embryonic development. Here, we report that the RNA-binding protein GLD-1 in C. elegans affects multiple microRNA pathways and interacts with proteins required for microRNA function. Using genome-wide RNAi screening, we found that nhl-2 and vig-1, two known modulators of miRNA function, genetically interact with GLD-1. gld-1 mutations enhance multiple phenotypes conferred by mir-35 and let-7 family mutants during somatic development. We used stable isotope labelling with amino acids in cell culture to globally analyse the changes in the proteome conferred by let-7 and gld-1 during animal development. We identified the histone mRNA-binding protein CDL-1 to be, in part, responsible for the phenotypes observed in let-7 and gld-1 mutants. The link between GLD-1 and miRNA-mediated gene regulation is further supported by its biochemical interaction with ALG-1, CGH-1 and PAB-1, proteins implicated in miRNA regulation. Overall, we have uncovered genetic and biochemical interactions between GLD-1 and miRNA pathways.
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