ArticleJournal of the Royal Society, Interface2014
Mechanosensitive channels and bacterial cell wall integrity: does life end with a bang or a whimper?
Article in Journal of the Royal Society, Interface, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- Lipid interactions and gating hysteresis suggest a physiological role for mechanosensitive channel YnaI.Nature communications · 2025Article
- Mechanical stability of individual bacterial cells under different osmotic pressure conditions: a nanoindentation study ofBeilstein journal of nanotechnology · 2025Article
- Antibiotic fosmidomycin protects bacteria from cell wall perturbations by antagonizing oxidative damage-mediated cell lysis.Frontiers in microbiology · 2025Article
- Observing mechanosensitive channels in action in living bacteria.Biophysical reports · 2024Article
- Mechanosensitive channel MscS is critical for termination of the bacterial hypoosmotic permeability response.The Journal of general physiology · 2023Article
- Quiescence ofMicroorganisms · 2023Article
- Detecting Bacterial Cell Viability in Few µL Solutions from Impedance Measurements on Silicon-Based Biochips.International journal of molecular sciences · 2021Article
- Understanding Beta-Lactam-Induced Lysis at the Single-Cell Level.Frontiers in microbiology · 2021Article
- Effects of Osmotic Stress and Sodium Hypochlorite on Endodontic Microbiota: An In-Vitro Study.European endodontic journal · 2020Article
- Bacterial nanotubes as a manifestation of cell death.Nature communications · 2020Article
- Interaction of the Mechanosensitive Channel, MscS, with the Membrane Bilayer through Lipid Intercalation into Grooves and Pockets.Journal of molecular biology · 2019Article
- Mechanics and Dynamics of Bacterial Cell Lysis.Biophysical journal · 2019Article
- Review
- Translation Stress Positively Regulates MscL-Dependent Excretion of Cytoplasmic Proteins.mBio · 2018Article
- Bacterial Cell Mechanics.Biochemistry · 2017Review
- Tension-activated channels in the mechanism of osmotic fitness inThe Journal of general physiology · 2017Article
- Maltodextrin enhances biofilm elimination by electrochemical scaffold.Scientific reports · 2016Article
- On the mobility, membrane location and functionality of mechanosensitive channels in Escherichia coli.Scientific reports · 2016Article
- Evaporation-induced stimulation of bacterial osmoregulation for electrical assessment of cell viability.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Mechanogated channels are fundamental components of bacterial cells that enable retention of physical integrity during extreme increases in cell turgor. Optical tweezers combined with microfluidics have been used to study the fate of individual Escherichia coli cells lacking such channels when subjected to a bursting stress caused by increased turgor. Fluorescence-activated cell sorting and electron microscopy complement these studies. These analyses show that lysis occurs with a high probability, but the precise path differs between individual cells. By monitoring the loss of cytoplasmic green fluorescent protein, we have determined that some cells release this protein but remain phase dark (granular) consistent with the retention of the majority of large proteins. By contrast, most cells suffer cataclysmic wall failure leading to loss of granularity but with the retention of DNA and overall cell shape (protein-depleted ghosts). The time span of these events induced by hypo-osmotic shock varies but is of the order of milliseconds. The data are interpreted in terms of the timing of mechanosensitive channel gating relative to osmotically induced water influx.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.