ArticleMolecular biology of the cell2014
Distinct roles of cell wall biogenesis in yeast morphogenesis as revealed by multivariate analysis of high-dimensional morphometric data.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Role of Pbr1, a putative oxidoreductase, in the ER quality control and folding of yeast Fks1 glucan synthase.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Coniontins, lipopetaibiotics active against Candida auris identified from a microbial natural product fractionation library.Nature communications · 2025Article
- Application of unimodal probability distribution models for morphological phenotyping of budding yeast.FEMS yeast research · 2024Article
- A deep semantic network-based image segmentation of soybean rust pathogens.Frontiers in plant science · 2024Article
- Analysis of the Localization ofInternational journal of molecular sciences · 2023Article
- Synergistic Antifungal Interactions between Antibiotic Amphotericin B and SelectedInternational journal of molecular sciences · 2023Article
- Jerveratrum-Type Steroidal Alkaloids Inhibit β-1,6-Glucan Biosynthesis in Fungal Cell Walls.Microbiology spectrum · 2022Article
- High-throughput platform for yeast morphological profiling predicts the targets of bioactive compounds.NPJ systems biology and applications · 2022Article
- Echinocandin Drugs Induce Differential Effects in Cytokinesis Progression and Cell Integrity.Pharmaceuticals (Basel, Switzerland) · 2021Article
- Defining Functions of Mannoproteins inJournal of fungi (Basel, Switzerland) · 2021Article
- Implications of maintenance of mother-bud neck size in diverse vital processes of Saccharomyces cerevisiae.Current genetics · 2019Article
- Antifungal effects of a 1,3,4-thiadiazole derivative determined by cytochemical and vibrational spectroscopic studies.PloS one · 2019Article
- Yeast species-specific, differential inhibition of β-1,3-glucan synthesis by poacic acid and caspofungin.Cell surface (Amsterdam, Netherlands) · 2018Article
- Functional annotation of chemical libraries across diverse biological processes.Nature chemical biology · 2017Article
- A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging.Annual review of physiology · 2017Review
- Unmasking fungal pathogens by studying MAPK-dependent cell wall regulation in Candida albicans.Virulence · 2016Article
- Single-cell phenomics in budding yeast.Molecular biology of the cell · 2015Review
- Microbiology: Fungus against the wall.Nature · 2015Article
- Plant-derived antifungal agent poacic acid targets β-1,3-glucan.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
The cell wall of budding yeast is a rigid structure composed of multiple components. To thoroughly understand its involvement in morphogenesis, we used the image analysis software CalMorph to quantitatively analyze cell morphology after treatment with drugs that inhibit different processes during cell wall synthesis. Cells treated with cell wall-affecting drugs exhibited broader necks and increased morphological variation. Tunicamycin, which inhibits the initial step of N-glycosylation of cell wall mannoproteins, induced morphologies similar to those of strains defective in α-mannosylation. The chitin synthase inhibitor nikkomycin Z induced morphological changes similar to those of mutants defective in chitin transglycosylase, possibly due to the critical role of chitin in anchoring the β-glucan network. To define the mode of action of echinocandin B, a 1,3-β-glucan synthase inhibitor, we compared the morphology it induced with mutants of Fks1 that contains the catalytic domain for 1,3-β-glucan synthesis. Echinocandin B exerted morphological effects similar to those observed in some fks1 mutants, with defects in cell polarity and reduced glucan synthesis activity, suggesting that echinocandin B affects not only 1,3-β-glucan synthesis, but also another functional domain. Thus our multivariate analyses reveal discrete functions of cell wall components and increase our understanding of the pharmacology of antifungal drugs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.