ArticleJournal of fluorescence2014
Complementary Methods of Processing diS-C3(3) Fluorescence Spectra Used for Monitoring the Plasma Membrane Potential of Yeast: Their Pros and Cons.
Article in Journal of fluorescence, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- The azole-resistance phenotype of aMicrobiology spectrum · 2026Article
- From the Metabolic Effects and Mechanism of Monovalent Cation Transport to the Actual Measurement of the Plasma Membrane Potential in Yeast.Journal of fungi (Basel, Switzerland) · 2025Review
- The Use of Thioflavin T for the Estimation and Measurement of the Plasma Membrane Electric Potential Difference in Different Yeast Strains.Journal of fungi (Basel, Switzerland) · 2023Article
- Identification of Antifungal HAntimicrobial agents and chemotherapy · 2017Article
- Computational models for monitoring the trans-membrane potential with fluorescent probes: the DiSCEuropean biophysics journal : EBJ · 2016Article
- Estimation of Candida albicans ABC Transporter Behavior in Real-Time via Fluorescence.Frontiers in microbiology · 2015Article
Corrections and comments
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Authors and funding
2 authors.
Funding
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Abstract
Carbocyanine dye diS-C3(3) was repeatedly employed in monitoring the plasma membrane potential of yeast and other living cells. Four methods of measuring and evaluating probe fluorescence signal were used in different studies, based on following fluorescence parameters: fluorescence intensity emitted within a certain spectral interval, F(580)/F(560) fluorescence emission ratio, wavelength of emission spectrum maximum, and the ratio of respective fluorescence intensities corresponding to the diS-C3(3) bound to cytosolic macromolecules and remaining dissolved in the aqueous cell medium (i.e., unbound, or free). Here we show that data corresponding to the three latter spectral assessments of diS-C3(3) accumulation in cells is mutually convertible, which means that their alternative use cannot lead to ambiguities in the interpretation of the results of biological experiments. On the other hand, experiments based on the effortless measurements of fluorescence intensities should be interpreted cautiously because controversial results can be obtained, depending on the particular choice of cell-to-dye concentration ratio and emission wavelength.
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