ArticleDevelopment (Cambridge, England)2014
The auto-inhibitory domain and ATP-independent microtubule-binding region of Kinesin heavy chain are major functional domains for transport in the Drosophila germline.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Tropomyosin 1-I/C coordinates kinesin-1 and dynein motors during oskar mRNA transport.Nature structural & molecular biology · 2024Article
- An RNA-based feed-forward mechanism ensures motor switching in oskar mRNA transport.The Journal of cell biology · 2023Article
- Kinesin-1 autoinhibition facilitates the initiation of dynein cargo transport.The Journal of cell biology · 2023Article
- Molecular basis of mRNA transport by a kinesin-1-atypical tropomyosin complex.Genes & development · 2021Article
- Sequences in the stalk domain regulate auto-inhibition and ciliary tip localization of the immotile kinesin-4 KIF7.Journal of cell science · 2021Article
- Swirling Instability of the Microtubule Cytoskeleton.Physical review letters · 2021Article
- Optical flow analysis reveals that Kinesin-mediated advection impacts the orientation of microtubules in theMolecular biology of the cell · 2020Article
- Article
- Autoinhibition of kinesin-1 is essential to the dendrite-specific localization of Golgi outposts.The Journal of cell biology · 2018Article
- Active diffusion and advection in Drosophila oocytes result from the interplay of actin and microtubules.Nature communications · 2017Article
- An RNA-binding atypical tropomyosin recruits kinesin-1 dynamically to oskar mRNPs.The EMBO journal · 2017Article
- A new isoform of Drosophila non-muscle Tropomyosin 1 interacts with Kinesin-1 and functions in oskar mRNA localization.Journal of cell science · 2016Article
- Microtubule-microtubule sliding by kinesin-1 is essential for normal cytoplasmic streaming in Drosophila oocytes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Article
- α-Spectrin and integrins act together to regulate actomyosin and columnarization, and to maintain a monolayered follicular epithelium.Development (Cambridge, England) · 2016Article
- JIP3 Activates Kinesin-1 Motility to Promote Axon Elongation.The Journal of biological chemistry · 2015Article
- Somatic insulin signaling regulates a germline starvation response in Drosophila egg chambers.Developmental biology · 2015Article
- Integrated regulation of motor-driven organelle transport by scaffolding proteins.Trends in cell biology · 2014Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The major motor Kinesin-1 provides a key pathway for cell polarization through intracellular transport. Little is known about how Kinesin works in complex cellular surroundings. Several cargos associate with Kinesin via Kinesin light chain (KLC). However, KLC is not required for all Kinesin transport. A putative cargo-binding domain was identified in the C-terminal tail of fungal Kinesin heavy chain (KHC). The tail is conserved in animal KHCs and might therefore represent an alternative KLC-independent cargo-interacting region. By comprehensive functional analysis of the tail during Drosophila oogenesis we have gained an understanding of how KHC achieves specificity in its transport and how it is regulated. This is, to our knowledge, the first in vivo structural/functional analysis of the tail in animal Kinesins. We show that the tail is essential for all functions of KHC except Dynein transport, which is KLC dependent. These tail-dependent KHC activities can be functionally separated from one another by further characterizing domains within the tail. In particular, our data show the following. First, KHC is temporally regulated during oogenesis. Second, the IAK domain has an essential role distinct from its auto-inhibitory function. Third, lack of auto-inhibition in itself is not necessarily detrimental to KHC function. Finally, the ATP-independent microtubule-binding motif is required for cargo localization. These results stress that two unexpected highly conserved domains, namely the auto-inhibitory IAK and the auxiliary microtubule-binding motifs, are crucial for transport by Kinesin-1 and that, although not all cargos are conserved, their transport involves the most conserved domains of animal KHCs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.