ArticleMethods (San Diego, Calif.)2014
Profiling post-transcriptionally networked mRNA subsets using RIP-Chip and RIP-Seq.
Article in Methods (San Diego, Calif.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
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- RNA-binding proteins in the mouse lens: Functional classifications, expression profiling, and interaction studies of Carhsp1 with crystallin mRNAs.Developmental biology · 2026Article
- Systematic identification of post-transcriptional regulatory modules.Nature communications · 2024Article
- FUS reads histone H3K36me3 to regulate alternative polyadenylation.Nucleic acids research · 2024Article
- SRSF10 regulates proliferation of neural progenitor cells and affects neurogenesis in developing mouse neocortex.iScience · 2023Article
- HIF-1-Induced hsa-miR-429: Understanding Its Direct Targets as the Key to Developing Cancer Diagnostics and Therapies.Cancers · 2023Review
- Native RNA Immunoprecipitation (RIP) for Precise Detection and Quantification of Protein-Interacting RNA.Methods in molecular biology (Clifton, N.J.) · 2023Article
- RNA-binding protein Elavl1/HuR is required for maintenance of cranial neural crest specification.eLife · 2022Article
- Quantitative analysis of protein-RNA interactions in fission yeast.STAR protocols · 2022Article
- Transcriptome-wide high-throughput mapping of protein-RNA occupancy profiles using POP-seq.Scientific reports · 2021Article
- HuR/ELAVL1 drives malignant peripheral nerve sheath tumor growth and metastasis.The Journal of clinical investigation · 2020Article
- Use of RNA Immunoprecipitation Method for DeterminingBiological procedures online · 2018Article
- Engineering Structurally Interacting RNA (sxRNA).Scientific reports · 2017Article
- Rules of RNA specificity of hnRNP A1 revealed by global and quantitative analysis of its affinity distribution.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- RNA-Seq methods for transcriptome analysis.Wiley interdisciplinary reviews. RNA · 2017Review
- Stem Cell Ribonomics: RNA-Binding Proteins and Gene Networks in Stem Cell Differentiation.Frontiers in molecular biosciences · 2015Review
- A Novel Role for VICKZ Proteins in Maintaining Epithelial Integrity during Embryogenesis.PloS one · 2015Article
- Library construction for next-generation sequencing: overviews and challenges.BioTechniques · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Post-transcriptional regulation of messenger RNA contributes to numerous aspects of gene expression. The key component to this level of regulation is the interaction of RNA-binding proteins (RBPs) and their associated target mRNA. Splicing, stability, localization, translational efficiency, and alternate codon use are just some of the post-transcriptional processes regulated by RBPs. Central to our understanding of these processes is the need to characterize the network of RBP-mRNA associations and create a map of this functional post-transcriptional regulatory system. Here we provide a detailed methodology for mRNA isolation using RBP immunoprecipitation (RIP) as a primary partitioning approach followed by microarray (Chip) or next generation sequencing (NGS) analysis. We do this by using specific antibodies to target RBPs for the capture of associated RNA cargo. RIP-Chip/Seq has proven to be is a versatile, genomic technique that has been widely used to study endogenous RBP-RNA associations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.