ReviewBiochemical Society transactions2013
The RNA- and DNA-targeting CRISPR-Cas immune systems of Pyrococcus furiosus.
Review in Biochemical Society transactions, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Histones direct site-specific CRISPR spacer acquisition in model archaeon.Nature microbiology · 2023Article
- Hyper-stimulation of Pyrococcus furiosus CRISPR DNA uptake by a self-transmissible plasmid.Extremophiles : life under extreme conditions · 2022Article
- Heavily Armed Ancestors: CRISPR Immunity and Applications in Archaea with a Comparative Analysis of CRISPR Types in Sulfolobales.Biomolecules · 2020Review
- Primed CRISPR DNA uptake in Pyrococcus furiosus.Nucleic acids research · 2020Article
- Regulation of the RNA and DNA nuclease activities required for Pyrococcus furiosus Type III-B CRISPR-Cas immunity.Nucleic acids research · 2020Article
- Review
- A journey down to hell: new thermostable protein-tags for biotechnology at high temperatures.Extremophiles : life under extreme conditions · 2020Article
- The nuts and bolts of the Haloferax CRISPR-Cas system I-B.RNA biology · 2019Article
- Tolerance of Sulfolobus SMV1 virus to the immunity of I-A and III-B CRISPR-Cas systems in Sulfolobus islandicus.RNA biology · 2019Article
- Article
- CRISPR RNA-guided DNA cleavage by reconstituted Type I-A immune effector complexes.Extremophiles : life under extreme conditions · 2019Article
- Application of Genome Editing Techniques in Immunology.Archivum immunologiae et therapiae experimentalis · 2018Review
- Cas4 Nucleases Define the PAM, Length, and Orientation of DNA Fragments Integrated at CRISPR Loci.Molecular cell · 2018Article
- Role of free DNA ends and protospacer adjacent motifs for CRISPR DNA uptake in Pyrococcus furiosus.Nucleic acids research · 2017Article
- Target DNA recognition and cleavage by a reconstituted Type I-G CRISPR-Cas immune effector complex.Extremophiles : life under extreme conditions · 2017Article
- Bipartite recognition of target RNAs activates DNA cleavage by the Type III-B CRISPR-Cas system.Genes & development · 2016Article
- The CRISPR-associated Csx1 protein of Pyrococcus furiosus is an adenosine-specific endoribonuclease.RNA (New York, N.Y.) · 2016Article
- DNA targeting by the type I-G and type I-A CRISPR-Cas systems of Pyrococcus furiosus.Nucleic acids research · 2015Article
- Diversity of CRISPR-Cas immune systems and molecular machines.Genome biology · 2015Review
- Three CRISPR-Cas immune effector complexes coexist in Pyrococcus furiosus.RNA (New York, N.Y.) · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Using the hyperthermophile Pyrococcus furiosus, we have delineated several key steps in CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) invader defence pathways. P. furiosus has seven transcriptionally active CRISPR loci that together encode a total of 200 crRNAs (CRISPR RNAs). The 27 Cas proteins in this organism represent three distinct pathways and are primarily encoded in two large gene clusters. The Cas6 protein dices CRISPR locus transcripts to generate individual invader-targeting crRNAs. The mature crRNAs include a signature sequence element (the 5' tag) derived from the CRISPR locus repeat sequence that is important for function. crRNAs are tailored into distinct species and integrated into three distinct crRNA-Cas protein complexes that are all candidate effector complexes. The complex formed by the Cmr [Cas module RAMP (repeat-associated mysterious proteins)] (subtype III-B) proteins cleaves complementary target RNAs and can be programmed to cleave novel target RNAs in a prokaryotic RNAi-like manner. Evidence suggests that the other two CRISPR-Cas systems in P. furiosus, Csa (Cas subtype Apern) (subtype I-A) and Cst (Cas subtype Tneap) (subtype I-B), target invaders at the DNA level. Studies of the CRISPR-Cas systems from P. furiosus are yielding fundamental knowledge of mechanisms of crRNA biogenesis and silencing for three of the diverse CRISPR-Cas pathways, and reveal that organisms such as P. furiosus possess an arsenal of multiple RNA-guided mechanisms to resist diverse invaders. Our knowledge of the fascinating CRISPR-Cas pathways is leading in turn to our ability to co-opt these systems for exciting new biomedical and biotechnological applications.
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