ArticlePloS one2013
Different host complement systems and their interactions with saliva from Lutzomyia longipalpis (Diptera, Psychodidae) and Leishmania infantum promastigotes.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
- Guianensin, a Blackfly Salivary Protein, Inhibits the Lectin Pathway of Complement.Journal of medicinal chemistry · 2025Article
- C-reactive protein binds to short phosphoglycan repeats ofFrontiers in immunology · 2023Article
- The host micro-RNA cfa-miR-346 is induced in canine leishmaniasis.BMC veterinary research · 2022Article
- Article
- Anti-complement activity in salivary glands and midgut of Chagas disease vector, Panstrongylus megistus (Hemiptera, Triatominae).Revista do Instituto de Medicina Tropical de Sao Paulo · 2019Article
- Second Blood Meal by FemaleBioMed research international · 2018Article
- Inhibition of the complement system by saliva of Anopheles (Nyssorhynchus) aquasalis.Insect biochemistry and molecular biology · 2018Article
- The Sand Fly Salivary Protein Lufaxin Inhibits the Early Steps of the Alternative Pathway of Complement by Direct Binding to the Proconvertase C3b-B.Frontiers in immunology · 2017Article
- Vaccinomics Approach to the Identification of Candidate Protective Antigens for the Control of Tick Vector Infestations andFrontiers in cellular and infection microbiology · 2017Article
- Saliva of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) inhibits classical and alternative complement pathways.Parasites & vectors · 2016Article
- An Inhibitor of the Alternative Pathway of Complement in Saliva of New World Anopheline Mosquitoes.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Inhibition of the classical pathway of the complement system by saliva of Amblyomma cajennense (Acari: Ixodidae).Experimental parasitology · 2016Article
- SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis.Scientific reports · 2016Article
- Escaping Deleterious Immune Response in Their Hosts: Lessons from Trypanosomatids.Frontiers in immunology · 2016Review
- Revisiting Trypanosoma rangeli Transmission Involving Susceptible and Non-Susceptible Hosts.PloS one · 2015Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLutzomyia longipalpis is the vector of Leishmania infantum in the New World, and its saliva inhibits classical and alternative human complement system pathways. This inhibition is important in protecting the insect´s midgut from damage by the complement. L. longipalpis is a promiscuous blood feeder and must be protected against its host's complement. The objective of this study was to investigate the action of salivary complement inhibitors on the sera of different host species, such as dogs, guinea pigs, rats and chickens, at a pH of 7.4 (normal blood pH) and 8.15 (the midgut pH immediately after a blood meal). We also investigated the role of the chicken complement system in Leishmania clearance in the presence and absence of vector saliva.
resultsThe saliva was capable of inhibiting classical pathways in dogs, guinea pigs and rats at both pHs. The alternative pathway was not inhibited except in dogs at a pH of 8.15. The chicken classical pathway was inhibited only by high concentrations of saliva and it was better inhibited by the midgut contents of sand flies. Neither the saliva nor the midgut contents had any effect on the avian alternative pathway. Fowl sera killed L. infantum promastigotes, even at a low concentration (2%), and the addition of L. longipalpis saliva did not protect the parasites. The high body temperature of chickens (40°C) had no effect on Leishmania viability during our assays.
conclusionSalivary inhibitors act in a species-specific manner. It is important to determine their effects in the natural hosts of Leishmania infantum because they act on canid and rodent complements but not on chickens (which do not harbour the parasite). Moreover, we concluded that the avian complement system is the probable mechanism through which chickens eliminate Leishmania and that their high body temperature does not influence this parasite.
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