ArticlemBio2013
Phage ϕC2 mediates transduction of Tn6215, encoding erythromycin resistance, between Clostridium difficile strains.
Article in mBio, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 91 citations in OpenAlex.
- From colonization to infection: Genomic evolution of Clostridioides difficile pathogenesis.Cell reports · 2026Review
- Review
- Integrated meta-omics reveals AFBMicrobiome · 2025Article
- Gut Virome and Aging: Phage-Driven Microbial Stability and Immune Modulation.Aging and disease · 2025Review
- The phi027 bacteriophage influences physiology and virulence of the lysogenic strain of Clostridioides difficile.Scientific reports · 2025Article
- Community-AcquiredInternational journal of molecular sciences · 2025Review
- Antibiotic Resistances of Clostridioides difficile.Advances in experimental medicine and biology · 2024Article
- Comparative genome analyses of clinical and non-clinicalFrontiers in microbiology · 2024Article
- Removal of mobile genetic elements from the genome ofFrontiers in microbiology · 2024Article
- Novel insights into phage biology of the pathogenFrontiers in microbiology · 2024Article
- Prophage Carriage and Genetic Diversity within Environmental Isolates ofInternational journal of molecular sciences · 2023Article
- Characterization and Genomic Analysis of a Novel Lytic Phage DCp1 againstInternational journal of molecular sciences · 2023Article
- Horizontal Gene Transfer of Antibiotic Resistance Genes in Biofilms.Antibiotics (Basel, Switzerland) · 2023Review
- Dominance of phage particles carrying antibiotic resistance genes in the viromes of retail food sources.The ISME journal · 2023Article
- Establishing genetic manipulation for novel strains of human gut bacteria.Microbiome research reports · 2023Review
- Diversity, Dynamics and Therapeutic Application ofViruses · 2022Review
- Characterization of a P1-bacteriophage-like plasmid (phage-plasmid) harbouringMicrobial genomics · 2022Article
- Similarities of P1-Like Phage Plasmids and Their Role in the Dissemination ofMicrobiology spectrum · 2022Article
- The gut virome: A new microbiome component in health and disease.EBioMedicine · 2022Review
- Antimicrobial resistance in Clostridioides difficile.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledIn this work, we show that Clostridium difficile phage ϕC2 transduces erm(B), which confers erythromycin resistance, from a donor to a recipient strain at a frequency of 10(-6) per PFU. The transductants were lysogenic for ϕC2 and contained the erm(B) gene in a novel transposon, Tn6215. This element is 13,008 bp in length and contains 17 putative open reading frames (ORFs). It could also be transferred at a lower frequency by filter mating. IMPORTANCE: Clostridium difficile is a major human pathogen that causes diarrhea that can be persistent and difficult to resolve using antibiotics. C. difficile is potentially zoonotic and has been detected in animals, food, and environmental samples. C. difficile genomes contain large portions of horizontally acquired genetic elements. The conjugative elements have been reasonably well studied, but transduction has not yet been demonstrated. Here, we show for the first time transduction as a mechanism for the transfer of a novel genetic element in C. difficile. Transduction may also be a useful tool for the genetic manipulation of C. difficile.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.