ArticleProceedings of the National Academy of Sciences of the United States of America2013
Genes involved in centrosome-independent mitotic spindle assembly in Drosophila S2 cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 64 citations in OpenAlex.
- A non-transcriptional mitotic function of POU/Oct factors ensures spindle assembly and chromosome segregation.Journal of cell science · 2026Article
- The role of chromosome/kinetochore-driven microtubule generation in Drosophila spindle assembly.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- Kif11-haploinsufficient oocytes reveal spatially differential requirements for chromosome biorientation.EMBO reports · 2025Article
- γ-TuRCs and the augmin complex are required for the development of highly branched dendritic arbors in Drosophila.Journal of cell science · 2024Article
- Ran-GTP assembles a specialized spindle structure for accurate chromosome segregation in medaka early embryos.Nature communications · 2024Article
- Microtubule nucleation for spindle assembly: one molecule at a time.Trends in biochemical sciences · 2023Review
- Mechanisms underlying spindle assembly and robustness.Nature reviews. Molecular cell biology · 2023Review
- Review
- Morphological growth dynamics, mechanical stability, and active microtubule mechanics underlying spindle self-organization.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.Cell reports · 2022Article
- Human centrosome organization and function in interphase and mitosis.Seminars in cell & developmental biology · 2021Review
- Stable kinetochore-microtubule attachments restrict MTOC position and spindle elongation in oocytes.EMBO reports · 2021Article
- Prc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes.Nature communications · 2020Article
- Centrosome Loss Triggers a Transcriptional Program To Counter Apoptosis-Induced Oxidative Stress.Genetics · 2019Article
- A novel microtubule nucleation pathway for meiotic spindle assembly in oocytes.The Journal of cell biology · 2018Article
- Review
- Ultrastructural analysis of mitotic Drosophila S2 cells identifies distinctive microtubule and intracellular membrane behaviors.BMC biology · 2018Article
- NEK7 regulates dendrite morphogenesis in neurons via Eg5-dependent microtubule stabilization.Nature communications · 2018Article
- 14-3-3 regulation of Ncd reveals a new mechanism for targeting proteins to the spindle in oocytes.The Journal of cell biology · 2017Article
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
Animal mitotic spindle assembly relies on centrosome-dependent and centrosome-independent mechanisms, but their relative contributions remain unknown. Here, we investigated the molecular basis of the centrosome-independent spindle assembly pathway by performing a whole-genome RNAi screen in Drosophila S2 cells lacking functional centrosomes. This screen identified 197 genes involved in acentrosomal spindle assembly, eight of which had no previously described mitotic phenotypes and produced defective and/or short spindles. All 197 genes also produced RNAi phenotypes when centrosomes were present, indicating that none were entirely selective for the acentrosomal pathway. However, a subset of genes produced a selective defect in pole focusing when centrosomes were absent, suggesting that centrosomes compensate for this shape defect. Another subset of genes was specifically associated with the formation of multipolar spindles only when centrosomes were present. We further show that the chromosomal passenger complex orchestrates multiple centrosome-independent processes required for mitotic spindle assembly/maintenance. On the other hand, despite the formation of a chromosome-enriched RanGTP gradient, S2 cells depleted of RCC1, the guanine-nucleotide exchange factor for Ran on chromosomes, established functional bipolar spindles. Finally, we show that cells without functional centrosomes have a delay in chromosome congression and anaphase onset, which can be explained by the lack of polar ejection forces. Overall, these findings establish the constitutive nature of a centrosome-independent spindle assembly program and how this program is adapted to the presence/absence of centrosomes in animal somatic cells.
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