ArticleDevelopment (Cambridge, England)2013
A small molecule screen identifies a novel compound that induces a homeotic transformation in Hydra.
Article in Development (Cambridge, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 41 citations in OpenAlex.
- Mechanical stretch regulates macropinocytosis inMolecular biology of the cell · 2024Article
- Nonlinear elasticity and short-range mechanical coupling govern the rate and symmetry of mouth opening inProceedings. Biological sciences · 2024Article
- Article
- On being a Hydra with, and without, a nervous system: what do neurons add?Animal cognition · 2023Review
- The Hippo pathway regulates axis formation and morphogenesis inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Cell Sorting in Hydra vulgaris Arises from Differing Capacities for Epithelialization between Cell Types.Current biology : CB · 2020Article
- The polymorphism of Hydra microsatellite sequences provides strain-specific signatures.PloS one · 2020Article
- Stem cell differentiation trajectories inScience (New York, N.Y.) · 2019Article
- Transgenesis in Hydra to characterize gene function and visualize cell behavior.Nature protocols · 2019Article
- Linalool acts as a fast and reversible anesthetic in Hydra.PloS one · 2019Article
- Applying evolutionary genetics to developmental toxicology and risk assessment.Reproductive toxicology (Elmsford, N.Y.) · 2017Review
- Dynamics of Mouth Opening in Hydra.Biophysical journal · 2016Article
- Insight into the molecular and functional diversity of cnidarian neuropeptides.International journal of molecular sciences · 2015Review
- Article
- Generation of transgenic Hydra by embryo microinjection.Journal of visualized experiments : JoVE · 2014Article
- PIWI proteins and PIWI-interacting RNAs function in Hydra somatic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Developmental processes such as morphogenesis, patterning and differentiation are continuously active in the adult Hydra polyp. We carried out a small molecule screen to identify compounds that affect patterning in Hydra. We identified a novel molecule, DAC-2-25, that causes a homeotic transformation of body column into tentacle zone. This transformation occurs in a progressive and polar fashion, beginning at the oral end of the animal. We have identified several strains that respond to DAC-2-25 and one that does not, and we used chimeras from these strains to identify the ectoderm as the target tissue for DAC-2-25. Using transgenic Hydra that express green fluorescent protein under the control of relevant promoters, we examined how DAC-2-25 affects tentacle patterning. Genes whose expression is associated with the tentacle zone are ectopically expressed upon exposure to DAC-2-25, whereas those associated with body column tissue are turned off as the tentacle zone expands. The expression patterns of the organizer-associated gene HyWnt3 and the hypostome-specific gene HyBra2 are unchanged. Structure-activity relationship studies have identified features of DAC-2-25 that are required for activity and potency. This study shows that small molecule screens in Hydra can be used to dissect patterning processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.