ArticlePharmaceutical research2013
Demonstration of ATP-dependent, transcellular transport of lipid across the lymphatic endothelium using an in vitro model of the lacteal.
Article in Pharmaceutical research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Bidirectional crosstalk between obesity and the lymphatic system: a novel pathway in obesity pathogenesis and treatment.International journal of obesity (2005) · 2026Review
- GPR182 and the reframing of lacteal chylomicron uptake.The Journal of clinical investigation · 2026Article
- GPR182 is a lipoprotein receptor for dietary fat absorption.The Journal of clinical investigation · 2026Article
- Understanding the Lymphatic System: Tissue-on-Chip Modeling.Annual review of biomedical engineering · 2025Review
- In Vitro Models of Blood and Lymphatic Vessels-Connecting Tissues and Immunity.Advanced biology · 2023Review
- Molecular Mechanisms Controlling Lymphatic Endothelial Junction Integrity.Frontiers in cell and developmental biology · 2020Review
- Application of microscale culture technologies for studying lymphatic vessel biology.Microcirculation (New York, N.Y. : 1994) · 2019Review
- Lymphatic Vessel Network Structure and Physiology.Comprehensive Physiology · 2018Review
- Insulin resistance disrupts cell integrity, mitochondrial function, and inflammatory signaling in lymphatic endothelium.Microcirculation (New York, N.Y. : 1994) · 2018Article
- Intestinal lymphatic vasculature: structure, mechanisms and functions.Nature reviews. Gastroenterology & hepatology · 2017Review
- Postprandial lymphatic pump function after a high-fat meal: a characterization of contractility, flow, and viscosity.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
- Lymphatic transport of exosomes as a rapid route of information dissemination to the lymph node.Scientific reports · 2016Article
- From sewer to saviour - targeting the lymphatic system to promote drug exposure and activity.Nature reviews. Drug discovery · 2015Review
- Microcirculation-on-a-Chip: A Microfluidic Platform for Assaying Blood- and Lymphatic-Vessel Permeability.PloS one · 2015Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
purposeThe lymphatic system plays crucial roles in tissue fluid balance, trafficking of immune cells, and the uptake of dietary lipid from the intestine. Given these roles there has been an interest in targeting lymphatics through oral lipid-based formulations or intradermal delivery of drug carrier systems. However the mechanisms regulating lipid uptake by lymphatics remain unknown. Thus we sought to modify a previously developed in vitro model to investigate the role of ATP in lipid uptake into the lymphatics.
methodsLymphatic endothelial cells were cultured on a transwell membrane and the effective permeability to free fatty acid and Caco-2 cell-secreted lipid was calculated in the presence or absence of the ATP inhibitor sodium azide.
resultsATP inhibition reduced Caco-2 cell-secreted lipid transport, but not dextran transport. FFA transport was ATP-dependent primarily during early periods of ATP inhibition, while Caco-2 cell-secreted lipid transport was lowered at all time points studied. Furthermore, the transcellular component of transport was highly ATP-dependent, a mechanism not observed in fibroblasts, suggesting these mechanisms are unique to lymphatics. Total transport of Caco-2 cell-secreted lipid was dose-dependently reduced by ATP inhibition, and transcellular lipoprotein transport was completely attenuated.
conclusionThe transport of lipid across the lymphatic endothelium as demonstrated with this in vitro model occurs in part by an ATP-dependent, transcellular route independent of passive permeability. It remains to be determined the extent that this mechanism exists in vivo and future work should be directed in this area.
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Registered trials
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