Evidence map›Paper›PMID 24251410›Full record

ArticleJournal of microscopy2014

Automatic segmentation of fluorescence lifetime microscopy images of cells using multiresolution community detection--a first study.

D Hu, P Sarder, P Ronhovde, S Orthaus, S Achilefu, Z Nussinov

Open access · greenAbstract read
In one paragraph

Article in Journal of microscopy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Review
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  4. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

D HuDepartment of Physics, Washington University, St. Louis, Missouri, USA.
P Sarder
P Ronhovde
S Orthaus
S Achilefu
Z Nussinov
Washington University in St. Louis · USPicoQuant (Germany) · DE

Funding

Task Specific Project 3: Diffuse Optical TomographyU54CA136398 · NCI · WASHINGTON UNIVERSITY · PI ACHILEFU, SAMUEL · 2008 to 2012
$6.0M
21ST CENTURY IMAGING SCIENCES: GRADUATE STUDENT TRAININGT32EB014855 · NIBIB · WASHINGTON UNIVERSITY · PI JOSEPH P CULVER, JOSEPH A O'SULLIVAN · 2012 to 2026
$3.1M
MOLECULAR PROBES AND METHODS FOR FLUORESCENCE LIFETIME TOMOGRAPHY AND MICROSCOPYR01EB008111 · NIBIB · WASHINGTON UNIVERSITY · PI ACHILEFU, SAMUEL · 2009 to 2012
$1.9M
NEAR INFRARED PH-SENSITIVE MOLECULAR PROBES FOR MICROSCOPY OF CELLS AND TISSUESR01EB007276 · NIBIB · WASHINGTON UNIVERSITY · PI ACHILEFU, SAMUEL · 2008 to 2011
$1.3M
Multiphoton microscopy using near infrared DyesR33CA123537 · NCI · WASHINGTON UNIVERSITY · PI ACHILEFU, SAMUEL · 2008 to 2010
$1.3M
NCI NIH HHS R33 CA123537NCI NIH HHS U54 CA136398NIBIB NIH HHS R01 EB007276NIBIB NIH HHS R01 EB008111NIBIB NIH HHS T32 EB014855PHS HHS HHSN268207000046C
6 · The paper itself

Abstract

Inspired by a multiresolution community detection based network segmentation method, we suggest an automatic method for segmenting fluorescence lifetime (FLT) imaging microscopy (FLIM) images of cells in a first pilot investigation on two selected images. The image processing problem is framed as identifying segments with respective average FLTs against the background in FLIM images. The proposed method segments a FLIM image for a given resolution of the network defined using image pixels as the nodes and similarity between the FLTs of the pixels as the edges. In the resulting segmentation, low network resolution leads to larger segments, and high network resolution leads to smaller segments. Furthermore, using the proposed method, the mean-square error in estimating the FLT segments in a FLIM image was found to consistently decrease with increasing resolution of the corresponding network. The multiresolution community detection method appeared to perform better than a popular spectral clustering-based method in performing FLIM image segmentation. At high resolution, the spectral segmentation method introduced noisy segments in its output, and it was unable to achieve a consistent decrease in mean-square error with increasing resolution.

Indexed as

Automation, LaboratoryCytological TechniquesImage Processing, Computer-AssistedMicroscopy, FluorescenceFluorescence lifetime imaging microscopymultiresolution community detectionspectral clustering

Identifiers

PMID24251410
PMCPMC3893052
OpenAlexW1884691121

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.