ArticlePloS one2013
Primary 1,25-dihydroxyvitamin D3 response of the interleukin 8 gene cluster in human monocyte- and macrophage-like cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Winter cholecalciferol supplementation at 55°N has little effect on markers of innate immune defense in healthy children aged 4-8 years: a secondary analysis from a randomized controlled trial.European journal of nutrition · 2019Trial
- Serum 25(OH)D3 level is associated with cognitive impairment in middle-aged and elderly patients with type 2 diabetes mellitus.Frontiers in endocrinology · 2026Article
- Fat-soluble Vitamins and Lung Cancer: Where We Are?Recent patents on anti-cancer drug discovery · 2025Review
- A Single Vitamin DNutrients · 2022Article
- Vitamin D Levels in Children with Recurrent Acute Tonsillitis in Jordan: A Case-Control Study.International journal of environmental research and public health · 2022Article
- Review
- Vitamin D and Beta-Glucans Synergically Stimulate Human Macrophage Activity.International journal of molecular sciences · 2021Article
- A hierarchical regulatory network analysis of the vitamin D induced transcriptome reveals novel regulators and complete VDR dependency in monocytes.Scientific reports · 2021Article
- Article
- Evolving Role of Vitamin D in Immune-Mediated Disease and Its Implications in Autoimmune Hepatitis.Digestive diseases and sciences · 2019Review
- Transcriptome analysis in primary colorectal cancer tissues from patients with and without liver metastases using next-generation sequencing.Cancer medicine · 2017Article
- 25-Hydroxyvitamin D and Peripheral Immune Mediators: Results from Two Nationwide Danish Pediatric Cohorts.Nutrients · 2017Article
- Hepatitis C infected patients need vitamin D3 supplementation in the era of direct acting antivirals treatment.World journal of gastroenterology · 2017Article
- Article
- Vitamin D interacts with Esr1 and Igf1 to regulate molecular pathways relevant to Alzheimer's disease.Molecular neurodegeneration · 2016Article
- Vitamin D in Autoimmunity: Molecular Mechanisms and Therapeutic Potential.Frontiers in immunology · 2016Review
- Vitamin D and respiratory tract infections in childhood.BMC infectious diseases · 2015Review
- Down-regulation of IL-8 by high-dose vitamin D is specific to hyperinflammatory macrophages and involves mechanisms beyond up-regulation of DUSP1.British journal of pharmacology · 2015Article
- Regulation of Dendritic Cell Function by Vitamin D.Nutrients · 2015Review
- Vitamin D and chronic hepatitis C: effects on success rate and prevention of side effects associated with pegylated interferon-α and ribavirin.International journal of clinical and experimental medicine · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome-wide analysis of vitamin D receptor (VDR) binding sites in THP-1 human monocyte-like cells highlighted the interleukin 8 gene, also known as chemokine CXC motif ligand 8 (CXCL8). CXCL8 is a chemotactic cytokine with important functions during acute inflammation as well as in the context of various cancers. The nine genes of the CXCL cluster and the strong VDR binding site close to the CXCL8 gene are insulated from neighboring genes by CCCTC-binding factor (CTCF) binding sites. Only CXCL8, CXCL6 and CXCL1 are expressed in THP-1 cells, but all three are up-regulated primary 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) target genes. Formaldehyde-assisted isolation of regulatory elements sequencing analysis of the whole CXCL cluster demonstrated 1,25(OH)2D3-dependent chromatin opening exclusively for the VDR binding site. In differentiated THP-1 cells the CXCL8 gene showed a 33-fold higher basal expression, but is together with CXCL6 and CXCL1 still a primary 1,25(OH)2D3 target under the control of the same genomic VDR binding site. In summary, both in undifferentiated and differentiated THP-1 cells the genes CXCL8, CXCL6 and CXCL1 are under the primary control of 1,25(OH)2D3 and its receptor VDR. Our observation provides further evidence for the immune-related functions of vitamin D.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.