ArticleIranian journal of pharmaceutical research : IJPR2013
Studying the Stability of S-Layer Protein of Lactobacillus Acidophilus ATCC 4356 in Simulated Gastrointestinal Fluids Using SDS-PAGE and Circular Dichroism.
Article in Iranian journal of pharmaceutical research : IJPR, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Functional Properties of Heat-Killed Lactic Acid Bacteria Isolated from Vietnamese Feces.Food science of animal resources · 2025Article
- Heat-killed probiotic Levilactobacillus brevis MKAK9 and its exopolysaccharide promote longevity by modulating aging hallmarks and enhancing immune responses in Caenorhabditis elegans.Immunity & ageing : I & A · 2024Article
- Surface layer proteins in species of the family Lactobacillaceae.Microbial biotechnology · 2023Article
- Ameliorative Effect of Surface Proteins of Probiotic Lactobacilli in Colitis Mouse Models.Frontiers in microbiology · 2021Article
- Extremophilic Microorganisms for the Treatment of Toxic Pollutants in the Environment.Molecules (Basel, Switzerland) · 2020Review
- Strategies to display heterologous proteins on the cell surface of lactic acid bacteria using as anchor the C-terminal domain of Lactobacillus acidophilus SlpA.World journal of microbiology & biotechnology · 2020Article
- The importance of the S-layer on the adhesion and aggregation ability of Lactic acid bacteria.Molecular biology reports · 2020Article
- Extractable Bacterial Surface Proteins in Probiotic-Host Interaction.Frontiers in microbiology · 2018Review
- Inhibition of H9N2 Virus Invasion into Dendritic Cells by the S-Layer Protein fromFrontiers in cellular and infection microbiology · 2016Article
- Role of S-layer proteins in bacteria.World journal of microbiology & biotechnology · 2015Review
- Precipitation Reaction of SDS and Potassium Salts in Flocculation of a Micronized Megestrol Acetate Suspension.Iranian journal of pharmaceutical research : IJPR · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Crystalline arrays of proteinaceous subunits forming surface layers (S-layers) are now recognized as one of the most common outermost cell envelope components of prokaryotic organisms. The surface layer protein of Lactobacillus acidophilus ATCC4356 is composed of a single species of protein of apparent molecular weight of 43-46 KDa. Considering the Lactobacillus acidophilus ATCC4356 having the S-layer is stable in harsh gastrointestinal (GI) conditions, a protective role against destructive GI factors which has been proposed for these nanostructures. It opens interesting perspectives in the using and development of this S-layer as a protective coat for oral administration of unstable drug nanocarriers. To achieve this goal, it is necessary to study the in-vitro stability of the S-layers in the simulated gastrointestinal fluids (SGIF). This study was planned to evaluate the in-vitro stability of the extracted S-layer protein of Lactobacillus acidophilus ATCC4356 in SGIF using it as a protective coat in oral drug delivery. Sodium dodecyl sulfate gel electrophoresis (SDS-PAGE) and circular dichroism (CD) spectroscopy were used to study the stability of the S-layer protein incubated in SGIF. Both the SDS-PAGE and CD spectra results showed that Lactobacillus acidophilus ATCC4356 S-layer protein is stable in simulated gastric fluid (SGF) with pH = 2 up to 5 min. It is stable in SGF pH = 3.2 and above it, with and without pepsin. It is also stable in all the simulated intestinal fluids. This S-layer is also stable in all of the simulated intestinal fluids.
Indexed as
Identifiers
24250671PMC3813368W3086119106What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.