ArticleIranian journal of pharmaceutical research : IJPR2013
Apoptosis Induction of Salvia chorassanica Root Extract on Human Cervical Cancer Cell Line.
Article in Iranian journal of pharmaceutical research : IJPR, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- The Effect ofMolecules (Basel, Switzerland) · 2024Article
- Assessment of Cytotoxic and Apoptotic Effects ofIranian journal of pharmaceutical research : IJPR · 2021Article
- An Insight into the Mechanism of Holamine- and Funtumine-Induced Cell Death in Cancer Cells.Molecules (Basel, Switzerland) · 2020Article
- Article
- In Vitro Antibacterial and Antiproliferative Potential ofPharmaceuticals (Basel, Switzerland) · 2020Article
- Article
- Auraptene-induced cytotoxicity mechanisms in human malignant glioblastoma (U87) cells: role of reactive oxygen species (ROS).EXCLI journal · 2019Article
- Cell Cycle Arrest and Apoptosis in HT-29 Cells Induced by Dichloromethane Fraction FromFrontiers in pharmacology · 2018Article
- Synthesis of Saccharumoside-B analogue with potential of antiproliferative and pro-apoptotic activities.Scientific reports · 2017Article
- Anticancer Properties of Chrysin on Colon Cancer Cells,Iranian journal of biotechnology · 2016Article
- Evaluation of the cytotoxic effects ofOncology letters · 2016Article
- Anti-tumor Activity of Ferulago angulata Boiss. Extract in Gastric Cancer Cell Line via Induction of Apoptosis.Iranian journal of pharmaceutical research : IJPR · 2014Article
- In-vitro Evaluation of Cytotoxic and Apoptogenic Properties of Sophora Pachycarpa.Iranian journal of pharmaceutical research : IJPR · 2014Article
- Growth Inhibition and Apoptosis Induction of Salvia chloroleuca on MCF-7 Breast Cancer Cell Line.Iranian journal of pharmaceutical research : IJPR · 2013Article
- Inhibitory Activity of Avicennia marina, a Medicinal Plant in Persian Folk Medicine, against HIV and HSV.Iranian journal of pharmaceutical research : IJPR · 2013Article
- Cytotoxic and Apoptotic Potential of Rheum turkestanicum Janisch Root Extract on Human Cancer and Normal Cells.Iranian journal of pharmaceutical research : IJPR · 2013Article
- Cytotoxic, antioxidant and antimicrobial activities and phenolic contents of eleven salvia species from iran.Iranian journal of pharmaceutical research : IJPR · 2013Article
- Cytotoxic evaluation of different fractions of Salvia chorassanica Bunge on MCF-7 and DU 145 cell lines.Research in pharmaceutical sciencesArticle
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salvia chorassanica Bunge is one of the Iranian endemic species of Salvia. There is not any reported literature on S. chorassanica. This study was designed to examine the in-vitro anti-proliferative and proapoptotic effects of the methanol extract of S. chorassanica and its fractions on HeLa cell line. Cells were cultured in EX-CELL®, an animal free medium specially designed for HeLa cell line and incubated with different concentrations of plant extracts. Cell viability was quantified by MTS assay. Apoptotic cells were determined using propidium iodide (PI) staining of DNA fragmentation by flow cytometry (sub-G1 peak). Activity of caspase -3, -8 and -9 was measured by the caspase colorimetric kit assay. S. chorassanica inhibited the growth of malignant cells and the CH2Cl2 fraction was determined as the most cytotoxic fraction in comparison with other fractions. The calculated IC50 values for methanol extract, n-hexane, CH2Cl2 and EtOAc fractions were 8.841, 5.45, 2.38, and 58.03 μg/mL, respectively. S. chorassanica induced a sub-G1 peak in the flow cytometry histogram of treated cells compared to control cells indicating that the cytotoxic mechanism is characterized by apoptosis induction. The activity of caspase-3 and 8 proteins in treated HeLa cells was significantly higher than that of the control while caspase-9 activity did not change significantly. Based on the result obtained from our study, the apoptosis pathway involved in S. chorassanica-induced cell death may be through the extrinsic pathway and it can be a novel promising candidate in the treatment of cancer.
Indexed as
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24250574PMC3813197What OpenQuestion holds
Registered trials
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